Clostridium sordellii is a spore-forming, obligately anaerobic, Gram-positive bacterium that can cause toxic shock syndrome after gynecological procedures. Although the incidence of C. sordellii infection is low, it is fatal in most cases. Since spore germination is believed to be the first step in the establishment of Bacilli and Clostridia infections, we analyzed the requirements for C. sorde...

Effective control of spore-forming bacilli begs suitable physical or chemical methods. While many spore inactivation techniques have been proven effective, electron beam (EB) irradiation has been frequently chosen to eradicate Bacillus spores. Despite its widespread use, there are limited data evaluating the effects of EB irradiation on Bacillus spores. To study this, B. atrophaeus spores were ...

This paper addresses the general concern in plant pathology that the introduction of quantitative resistance in the landscape can lead to increased pathogenicity. Hereto, we study the hypothetical case of a quantitative trait loci (QTL) acting on pathogen spore production per unit lesion area. To regain its original fitness, the pathogen can break the QTL, restoring its spore production capacit...

AIMS To maximize spore production by Bacillus amyloliquefaciens B128, and its antagonism to the fungal pathogen Botrytis elliptica B061. METHODS AND RESULTS In the 5-l stirred-tank bioreactor (STR), with the 0.5 vvm aeration rate, an agitation rate of 200 rev min(-1) significantly enhanced the spore yield compared to the same in 300 rev min(-1) cultivations. In a 20-l airlift bioreactor (ALR)...

Six strains of mycobacteria,-three human strains, Saranac H-37, T. S., and No. 90, a bovine strain, B-1, a smegma strain, No. 74, and a Saranac strain of B. phlei,-have been made to grow as non-acid-fast organisms by the addition to the culture media of a filtered extract of the chromogenic H-37 strain of B. tuberculosis. The action of the extract produced acceleration of growth of the treated ...

The bacterial spore can rapidly convert from a dormant to a fully active cell. Here we study this remarkable cellular transition in Bacillus subtilis and reveal the identity of the newly synthesized proteins throughout spore revival. Our analysis uncovers a highly ordered developmental program that correlates with the spore morphological changes and reveals the spatial and temporal molecular ev...

Endospores of Bacillus subtilis have been used extensively as a platform for recombinant protein display for nearly two decades. Main use of the spore surface display system is generation of oral vaccines against many human and animal pathogens. Formulation of oral vaccine based on spores is an attractive approach to alternative vaccination due to the timesaving and relative easiness of product...

Airborne biological particles are present in every type of environment. Different types of geographical localities have different type of airspora, which affect human health. The current study is conducted for the first time to identify the airborne fungal spores from Hyderabad: Tando-Jam, Sindh. For this purpose, Burkard’s 7-Days recording volumetric spore trap was used for a period of one yea...

During yeast sporulation, internal membrane synthesis ensures that each haploid nucleus is packaged into a spore. Prospore membrane formation requires Spo14p, a phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2]-stimulated phospholipase D (PLD), which hydrolyzes phosphatidylcholine (PtdCho) to phosphatidic acid (PtdOH) and choline. We found that both meiosis and spore formation also require ...

At a concentration of 0.025% (w/v) chitosan-chloride inhibited spore germination of Botrytis cinerea, Penicillium expansum, and Candida saitoana. In contrast, at 0.5% (w/v) glycolchitosan inhibited spore germination of B. cinerea and P. expansum, but not the growth of C. saitoana in vitro or in apple wounds. The combination of C. saitoana with 0.2% glycolchitosan was more effective in controlli...