Simple sequence repeats (SSRs) or microsatellites are very useful molecular markers, owing to their locus-specific codominant and multiallelic nature, high abundance in the genome, and high rates of transferability across species. The soybean aphid (Aphis glycines Matsumura) has become the most damaging insect pest of soybean (Glycine max (L.) Merr.) in North America, since it was first found i...

This study aimed to evaluate the genetic diversity and identify potato cultivars by RAPD and SSR markers. The genomic DNA of 16 potato cultivars was amplified with 25 RAPD primers that generated 92 polymorphic bands and 20 SSR primer pairs that produced 136 polymorphic bands. The dendrograms generated by cluster analysis distinguished the cultivars genetically although the dendrograms were not ...

Although microsatellites or simple sequence repeats (SSRs) have become a popular tool in genetic mapping and gene flow studies, their utility is limited due to paucity of information about DNA sequences in plants. We tested the utility of microsatellite markers characterized for the tropical tree Pithecellobium elegans as a genetic tool for related species. The results indicate that SSR loci ar...

Simple sequence repeats (SSRs, also known as microsatellites) are known to be mutational hotspots in genomes. DNA rearrangements have also been reported to accompany allopolyploidization. A study of the effect of allopolyploidization on SSR mutation is therefore important for understanding the origin and evolutionary dynamics of SSRs in allopolyploids. Three synthesized double haploid (SynDH) p...

A total of 2,970 EST-SSRs (2.38%) were identified by transcriptome sequencing of clam Meretrix meretrix (751,970 reads, ~310.82 Mbp), using 454 Genome Sequencer FLX next-generation sequencing platform. Dinucleotide SSR was the dominant repeat type (40.2%), followed by trinucleotide (37.8%), tetranuleotide (12.0%) and pentanucleotide (2.0%) SSR. The dominant repeat motif was AT (71.3%) in the di...

Transfer of molecular markers is widely used in conservation genetic studies. We investigated the transferability of simple sequence repeat (SSR) markers developed for Enterolobium cyclocarpum to E. contortisiliquum, a tropical tree widely distributed in dry forests. A set of 9 evaluated SSR markers were amplified in E. contortisiliquum and the degree of polymorphism was assessed in 8 tre...

Forty-four soybean genotypes with different photoperiod response were selected after screening of 1000 soybean accessions under artificial condition and were profiled using 40 SSR and 5 AFLP primer pairs. The average polymorphism information content (PIC) for SSR and AFLP marker systems was 0.507 and 0.120, respectively. Clustering of genotypes was done using UPGMA method for SSR and AFLP and c...

Surveillance videos of coal mining faces have close relation to the safety of coal miners and mining efficiency. However, surveillance videos are always disturbed by some severe conditions such as atomization, low illumination, glare, and so on. Therefore, this paper proposed a hybrid algorithm (SSR-BF) based on the integration of single-scale Retinex (SSR) and bilateral filtering (BF) to enhan...

PREMISE OF THE STUDY Bombax ceiba (Malvaceae), commonly known as silk cotton tree, is a multipurpose tree species of tropical forests. Novel expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed and characterized for the species using transcriptome analysis. METHODS AND RESULTS A total of 33 new EST-SSR markers were developed for B. ceiba, of which 13 showed polymorph...

Polymerase chain reaction (PCR) provides a foundation for simple sequence repeat molecular marker-assisted selection (SSR MAS) in soybean. This PCR system and its various conditions have been optimized by many researchers. However, current research on the optimization of the PCR system focuses on double-primer PCR products. We compared single- and double-SSR primer PCR products from 50 soybean ...