Eosin Nigrosin staining for assessment of sperm vitality is an essential component basic semen analysis as it helps differentiate between dead and immotile sperms, has clinical implications in terms patient treatment follow up. This technique involves minimal use reagents simple procedural steps. Standardization the same pertinent to warrant accurate reproducible results medical laboratories, e...

herpetosiphon giganteus is a filamentous gliding bacterium. gliding motility is the movement of the cells over surfaces without the aid of flagella. the mechanism responsible for bacterial gliding motility has not been known and there are only a few data on herpetosiphon giganteus. the aim of this study was to observe the ultrastructure and negative staining of isolated strains of herpetosiphon...

materials and methods a total of 228 fecal samples were collected from residents of rural areas of hamadan, west of iran. each fecal sample was divided into two parts, one kept frozen at -20˚c for ag-capture elisa and the other in 10% formalin for the ap staining method. cryptosporidium ag-detection elisa procedure was performed according to the manual of the manufacturer. the preserved samples...

background and objective: tuberculosis is still a major health problem, involving about 1/3 of the world´s population. diagnosis is difficult when we only use ziehl-neelson staining. many cases may be missed. a more rapid and sensitive diagnostic method is necessary. pcr may be helpful. the aim of this study was to compare pcr, zieh-neelsen staining and histopathologic findings in diagnosis of ...

This is the report on a patient with chronic diarrhea caused by microsporidia. He is married, infected with HIV and has low CD4 cell count. The diagnosis was established through stool parasite search using concentration methods and Gram-chromotrope staining technique. Ileum biopsy was also performed in this case. The etiological diagnosis may be established in a clinical laboratory, by chromotr...

Fluorescent staining of the cyanoacrylate developed prints was known in the early 1980’s. Initially this technique could only be used by labs equipped with a laser, like the argon ion laser, even today an expensive light source. With the availability of cheaper light sources, most of them specially built for the forensic community, fluorescent staining of prints developed with cyanoacrylate bec...