Objective: Evaluation of the role of polymerase chain reaction (PCR) for the detection of Mycobacterium tuberculosis (MTB) DNA as a diagnostic aid in cutaneous tuberculosis. Study Design: Descriptive study. Duration and place of study: TB reference laboratory, PHLS, Ahvaz, Iran from May 2001 to December 2001. Patients & Methods: Thirty formalin-fixed, paraffin-embedded samples belonging to 28 p...

ObjectivesOne of the problems encountered in malaria control and elimination is inaccurate diagnosis, resulting from degree sensitivity different diagnostic tools. Even though microscopy remains gold standard for more sensitive robust tools such as polymerase chain reactions (PCR) are used research settings to monitor interventions track sub-microscopic infections due some drawbacks microscopy....

ProbeLibrary, a fast, specific and flexible format for quantitative real-time PCR, is described. The ProbeLibrary concept is based on the fact that just 90 short probes provide transcriptome-wide coverage in most organisms. These short probes are highly specific and possess the high melting temperature (Tm) required for real-time PCR owing to the incorporation of locked nucleic acid (LNA). The ...

background: methicillin resistant staphylococcus aureus (mrsa) has been emerged as a nosocomial and community acquired pathogen worldwide. there are many challenges for laboratory detection of mrsa. the aim of this study was to compare different phenotypic methods with pcr based method as a gold standard for detection of meca gene. methods: a total of 220 clinical isolates of s. aureus which we...

OBJECTIVES CTX-M extended-spectrum beta-lactamases (ESBLs) are emerging worldwide. Fast and reliable detection techniques may become mandatory for implementing proper treatment and infection control measures. Here, a bla(CTX-M)-specific LightCycler real-time PCR (LC-PCR) assay based on hybridization probes was developed. METHODS Urine samples positive for Gram-negative bacilli as revealed by ...

PCR is an exquisitely sensitive method for detection of minute amounts of target DNA. The exponential nature of DNA amplification, however, is prone to burden the experimental data with significant standard error due to the tube-to-tube inherent variations in amplification efficiency. Therefore the reliable quantitation of DNA or RNA templates by PCR necessitates introduction of an internal sta...

Typically, population-based sequencing of HIV does not detect minority variants present at levels below 20-30%. Single genome amplification (SGA) and sequencing improves detection, but it requires many PCRs to find the optimal terminal dilution to use. A novel method for guiding the selection of a terminal dilution was developed and compared to standard methods. A quantitative real-time PCR (qR...

Check out our Selection Guide! PCR RNA DNA LAMP KitsOne Tube For Superior Reproducibility. From fish tissues or fluids using a nested PCR primer set. The use of trade, firm, or corporation names in this protocol is for the information and.jor consideration in designing a nested PCR protocol compatible with quantitation is to assure that the maximum concentration of PCR products produced by the....

Background: COVID-19 affects different people in ways. The illness varies from mild to acute. Mild is treated even without hospitalization. RT-PCR one of the main techniques, which are used diagnose COVID-19, but this paper, we have investigated that Chest CT a more efficient alternative option RT-Polymerase Chain Reaction. purpose our study importance chest comparis...

OBJECTIVE To identify transcriptionally active open reading frames (ORFs), predicted by bioinformatics, within RD1 genomic segment of Mycobacterium tuberculosis using reverse transcription-polymerase chain reaction (RT-PCR). MATERIALS AND METHODS M. tuberculosis H37Rv was grown in Middlebrook 7H9 medium for 8 weeks and total RNA was isolated using standard procedures. The cDNA was synthesized...