To facilitate genetic studies of Escherichia coli, we constructed a complete set of mobile plasmid clones of intact open reading frames (ORFs). Their expression is strictly controlled by Ptac / lacI(q). The plasmids carrying each ORF were introduced into an F+ recA strain and stored in 96-well microtiter plates. In this way, 96 clones can be transferred simultaneously to F- bacteria using the c...

The thirst from which patients suffer after abdominal operations is so well known that I need not enlarge upon it. The cause of it, too, is sufficiently obvious. In such cases, fluids by the mouth are for a variable number of days contra-indicated, in order to obviate or minimize chloroform sickness, lest the consequent retching might strain the recent abdominal wound, and also in many cases to...

bioassays were conducted to assess the contact toxicity of two plant essential oils against adults of sitophilus granarius (l.) and tribolium confusum jacquelin du val. essential oils were extracted from seeds of carum copticum l. and cuminum cyminum l. contact toxicity was assessed by topical application and adult mortality was counted 1, 2, 6 and 24 h after exposure. bioassays were carried ou...

Lipid droplets store neutral lipids, primarily triacylglycerol and steryl esters. Seipin plays a role in lipid droplet biogenesis is thought to determine the site of size newly formed droplets. Here we show seipin-independent pathway biogenesis. In silico vitro experiments reveal that retinyl esters have intrinsic propensity sequester nucleate bilayers. Production mammalian yeast cells do not n...

Athermal random fiber networks are usually modeled by representing each fiber as a truss, a Euler-Bernoulli or a Timoshenko beam, and, in the case of cross-linked networks, each cross-link as a pinned, rotating, or welded joint. In this work we study the effect of these various modeling options on the dependence of the overall network stiffness on system parameters. We conclude that Timoshenko ...