Microsatellites are abundant in eukaryotic genomes and have high rates of strand slippage-induced repeat number alterations. They are popular genetic markers, and their mutations are associated with numerous neurological diseases. However, the minimal number of repeats required to constitute a microsatellite has been debated, and a definition of a microsatellite that considers its mutational be...

as a case study, the genotypic polymorphism of the iranian material of diploid triticum was screened based on internal transcribed spacer 2 (its2) of the ribosomal dna (rdna) using single strand conformation polymorphism (sscp) analysis. this is a simple and cost effective technique for genotyping and investigating the allelic polymorphism among plant populations. performing sscp analysis among...

PURPOSE To investigate the degree of heteroplasmy of the 11778 mtDNA mutation in Chinese patients with Leber's hereditary optic neuropathy (LHON). METHODS Seventeen Chinese Leber's pedigrees, including 24 patients, 17 unaffected maternal lineages, 4 internal controls, and 6 unrelated controls, were screened for the 11778 mtDNA mutation. This was carried out by analysis of the restriction frag...

Changes in molecular conformation and its relationship with crystal polymorphism have been well documented previous work. To the best of our knowledge, however, effect solvate formation on has never explored. Using Cambridge Structural Database combination modeling, we provide insights into impact adopted by a compound whether such is different to that found neat form(s). Typically, more flexib...

In the otherwise highly conserved NMR structures of cellular prion proteins (PrP(C)) from different mammals, species variations in a surface epitope that includes a loop linking a β-strand, β2, with a helix, α2, are associated with NMR manifestations of a dynamic equilibrium between locally different conformations. Here, it is shown that this local dynamic conformational polymorphism in mouse P...

BACKGROUND Pathogenic mutations in the presenilin 1 (PS1) gene leading to early-onset Alzheimer disease have been described in various populations. The different mutations are not distributed randomly in the PS1 protein but are clustered in some PS1 exons. OBJECTIVE To screen the PS1 gene in search of a potential mutation in a Spanish family with early-onset Alzheimer disease. METHODS Singl...

We have analyzed previously three representative p53 single-point mutations by capillary-electrophoresis single-strand conformation polymorphism (CE-SSCP). In the current study, we compared our CE-SSCP results with the potential secondary structures predicted by an RNA/DNA-folding algorithm with DNA energy rules, used in conjunction with a computer analysis workbench called STRUCTURELAB. Each o...

Association of genetic polymorphism in the calpastatin (CAST) gene with average daily gain was examined in Iranian purebred Kurdi sheep. The genotypes for CAST were determined by the PCR-SSCP method. Blood samples were collected from 84 purebred Kurdi sheep belonging to the Kurdi Breeding Station located in the Khorasan province, north-east of Iran. Extraction of genomic DNA was based on the Gu...