The tlrB gene from Streptomyces fradiae has been cloned and used to construct bifunctional Streptomyces-Escherichia coli shuttle vectors carrying the antibiotic resistance genes to kanamycin-neomycin, thiostrepton and tylosin as selection markers. In the same way, the tlrB gene was subcloned in plasmids including the apramycin resistance gene and the oriT sequence from the plasmid pSET152 to fa...

It is well knowni that the genus Pseudomonas comllpirises several p)igment-producing species, an(l that in all cases in whiich the constitution of these pigments lhais l)een estal)lishedl thlehave leen found to l)e plhenlzine (lerivatives. Wrede and Strack (1929) were the first to prove this foI pyocyanine, the pigment produce(l by Pseudomonas aeruginosa (P. pyocyanea). Pyocvanine is now usuall...

A novel endophytic actinomycete, designated strain NEAU-LZS-5(T), was isolated from the leaf of candelabra aloe (Aloe arborescens Mill) and characterized using a polyphasic approach. Analysis of the 16S rRNA gene sequence showed that strain NEAU-LZS-5(T) belongs to the genus Streptomyces and exhibited 99.51 and 97.37 % similarity to Streptomyces sedi YIM 65188(T) and Streptomyces specialis GW41...

Traditional three-domain fungal and bacterial laccases have been extensively studied for their significance in various biotechnological applications. Growing molecular evidence points to a wide occurrence of more recently recognized two-domain laccase-like multicopper oxidase (LMCO) genes in Streptomyces spp. However, the current knowledge about their ecological role and distribution in natural...

INTRODUCTION Streptomyces spp. are aerobic, Gram-positive bacteria of the order Actinomycetales, known for their ability to produce antimicrobial molecules such as streptomycin. Pneumonia due to Streptomyces is considered to be rare and limited to immunocompromised patients. Streptomyces spp. are only rarely associated with invasive systemic infections. To our knowledge, we report the first doc...

The bacterium Pseudomonas aureofaciens 63-28 is antagonistic to several plant pathogenic fungi, including Pythium spp. The bacterium produced at least four antifungal metabolites active against Pythium ultimum and Phytophthora cryptogea when tested in culture for antifungal activity. Two of these compounds were identified as the novel butyrolactones (Z)-4-hydroxy-4-methyl-2-(1-hexenyl)-2-buteno...

The paulomycins are a group of glycosylated compounds featuring a unique paulic acid moiety. To locate their biosynthetic gene clusters, the genomes of two paulomycin producers, Streptomyces paulus NRRL 8115 and Streptomyces sp. YN86, were sequenced. The paulomycin biosynthetic gene clusters were defined by comparative analyses of the two genomes together with the genome of the third paulomycin...

Streptomyces albus is the type species of the genus Streptomyces. Here, we report the draft genome sequence of S. albus strain NBRC 13014(T). The genome contains at least seven orphan polyketide synthase and nonribosomal peptide synthetase gene clusters. The genome sequence will also serve as a valuable reference for Streptomyces taxonomy.

It has long been thought that the life cycle of Streptomyces bacteria encompasses three developmental stages: vegetative hyphae, aerial hyphae and spores. Here, we show interactions between Streptomyces and fungi trigger a previously unobserved mode of Streptomyces development. We term these Streptomyces cells 'explorers', for their ability to adopt a non-branching vegetative hyphal conformatio...

We have demonstrated that the bldB gene of Streptomyces coelicolor is required for the formation of aerial hyphae and the synthesis of antibiotics. We also found that BldB forms a higher-order complex (most likely a dimer) and that amino acid residues 20 to 78 are important for this interaction. This region is conserved in the BldB family, suggesting that dimer formation may be a common feature...