Nerve growth factor (NGF), which has a tertiary structure based on a cluster of 3 cystine disulfides and 2 very extended, but distorted beta-hairpins, is the prototype of a larger family of neurotrophins. Prior to the availability of cloning techniques, the mouse submandibular gland was the richest source of NGF and provided sufficient material to enable its biochemical characterization. It bin...

The phenazines are a class of over 150 nitrogen-containing aromatic compounds of bacterial and archeal origin. Their redox properties not only explain their activity as broad-specificity antibiotics and virulence factors but also enable them to function as respiratory pigments, thus extending their importance to the primary metabolism of phenazine-producing species. Despite their discovery in t...

The rapid provision of purified native protein underpins both structural biology and the development of new biopharmaceuticals. The dominance of Escherichia coli as a cellular biofactory depends on technology for solubilizing and refolding proteins that are expressed as insoluble inclusion bodies. Such technology must be scale invariant, easily automated, generic for a broad range of similar pr...

Bacterial chaperonin, GroEL, together with its co-chaperonin, GroES, facilitates the folding of a variety of polypeptides. Experiments suggest that GroEL stimulates protein folding by multiple cycles of binding and release. Misfolded proteins first bind to an exposed hydrophobic surface on GroEL. GroES then encapsulates the substrate and triggers its release into the central cavity of the GroEL...

Cutting maneuvers (CM) are frequently executed in sports. This task is relevant as it is a key ability for team sports players (TSP) but also bears the risk of ACL tears. An analysis of the movement strategies (MS) and musculoskeletal loads (MSL) occurring during CMs could reveal factors for efficient CMs and major triggers governing the occurrence of ACL tears. This study investigated the MS a...

Numerous scholars and practitioners claim that motivational factors can facilitate successful knowledge sharing. However, little empirical research has been conducted examining the different kinds of motivation (extrinsic and intrinsic) used to explain employee knowledge sharing behaviors. By integrating a motivational perspective into the theory of reasoned action (TRA), this study examines th...

Measuring the effectiveness of corporate environmental reports, it being highly qualitative and less regulated, is often considered as a daunting task. The task becomes more complex if comparisons are to be performed. This study is undertaken to overcome the physical verification problems by implementing data mining technique. It further explores on the effectiveness by performing exploratory a...

Pain in the upper extremity, particufariy in the neck and shoulder region is a major source of work place injuries in i ndust ri esThe occurrence of such occupational injuries is also on the rise. Shoulder and neck pain has been associateâ with awkward and statïc postures, repetitive a m movements and lack of rest A W a r d working postures at the trunk, neck and shoulderç may be caused by a nu...

This paper empirically investigates whether information technology (IT) strategies, business strategies, and divisions are aligned to meet overall business goals for Korean Small and medium-sized enterprises (SMEs), based on structure based Strategic Alignment Model, and make comparison with those of Japanese SMEs. Using 2,869 valid responses of Korean Human Capital Corporate Panel survey, a re...

A reduction in academic achievement over the course of adolescence has been observed. School failure is characterized by difficulties to teaching school goals. A variety of other behavioral problems are often associated with school failure. Child-to-parent violence has been associated with different school problems. The main objective of current study was to examine the contribution of family v...