Fourteen Escherichia coli O157 strains isolated from cattle and pigs in Poland and in Germany were investigated, using PCR, for the genetic markers associated with Shiga toxin-producing E. coli (STEC). Only two strains, both of cattle origin, were positive for the fliC (H7) gene and could be classified as O157 : H7. Nine isolates had stx shiga toxin genes, either stx1 (1 strain), stx2 (4 isolat...

1 MD (Resident ENT Physician at EPM-UNIFESP). 2 MD, Head and Neck Surgeon (MD, Head and Neck Surgeon at EPM-UNIFESP). 3 MD, ENT, Head and Neck Surgeon (MD, ENT, Head and Neck Surgeon at EPM UNIFESP). 4 MSc on Pathology at EPM-UNIFESP (Professor in the Department of Pathology at EPM-UNIFESP). 5 Adjunct Professor in the Department of Otorhinolaryngology and Head and Neck Surgery at EPM-UNIFESP (V...

Enterohemorrhagic Escherichia coli (EHEC) O26 infections cause severe human diseases such as hemolytic uremic syndrome and encephalopathy, and is the predominant serogroup among non-O157 EHEC in many countries. Shiga toxin (Stx), which consists of two distinct types (Stx1 and Stx2), plays a central role in EHEC pathogenesis. The major stx gene type in EHEC O26 strains is stx1, although isolates...

BACKGROUND & OBJECTIVE An oedema outbreak occurred in a Guwahati pig farm. Escherichia coli isolates from different necropsy samples collected from the dead piglets with oedema were characterized to confirm the virulence. METHODS Haemolytic E. coli isolates recovered from liver, lung and intestine of pigs with oedema were examined for presence of genes encoding pathogroups such as enteropatho...

Hemolytic uremic syndrome (HUS) from enterohemorrhagic Escherichia coli infection is a leading cause of kidney failure in otherwise healthy U.S. children. The bacterial Shiga toxins (Stx) induce the characteristic coagulopathy of HUS, but the damage to toxin-receptor expressing cells and organ injury due to ischemia likely also releases inflammatory damage-associated molecular patterns (DAMPs),...

BACKGROUND & OBJECTIVES Verotoxigenic Escherichia coli are important serotypes of enterohaemorrhagic E. coli (EHEC) subgroup that cause attaching and effacing lesions in enterocytes by producing verotoxins or shiga-like toxins resulting in haemorrhagic colitis (HC) and haemolytic uremic syndrome (HUS). The aim of this study was to detect these serotypes specially E. coli O157:H7 in stool sample...

Associations between known or putative virulence factors of Shiga toxin-producing Escherichia coli and disease in humans were investigated. Univariate analysis and multivariate logistic regression analysis of a set of 237 isolates from 118 serotypes showed significant associations between the presence of genes for intimin (eae) and Shiga toxin 2 (stx2) and isolates from serotypes reported in hu...

Shiga toxin producing bacteria are potential causes of serious human disease such as hemorrhagic colitis, severe inflammations of ileocolonic regions of gastrointestinal tract, thrombocytopenia, septicemia, malignant disorders in urinary ducts, hemolytic uremic syndrome (HUS). Shiga toxin 1 (stx1), shiga toxin 2 (stx2), or a combination of both are responsible for most clinical symptoms of thes...

In this study, which was conducted for the first time in Balıkesir, it purposed to determine existense, virulence factors and antibiotic susceptibility of Escherichia coli O157, is zoonotic ruminant feces feed, pathogen Listeria monocytogenes, causes diseases humans animals, use these results as epidemiological data our province, region country. Feces animal feed samples were analyzed simultane...

Escherichia coli is one of the most important bacteria associated with consumption meat products causing human disease, bovines as main reservoir for this organism. Microbiological quality raw sampled at slaughterhouses was evaluated by measuring presence serogroups that produce Shiga toxin (referred to STEC), including both O157:H7 and non-O157 strains can cause Hemolytic Uremic Syndrome (HUS)...