Despite the great advances in solid-phase peptide synthesis (SPPS), incorporation of certain functional groups into sequences is restricted by compatibility building blocks with conditions used during SPPS. In particular, introduction highly reactive modern bioorthogonal reactions peptides remains elusive. Here, we present an optimized synthetic protocol enabling installation two strained dieno...

Polyetheretherketone (PEEK) has favorable biomechanical properties to be used as an implant material. Unfortunately, it is hydrophobic and does not promote cellular adhesion, which could result in poor integration with bone tissue. Bio-functionalization of PEEK surface osteogenic peptides derived from extracellular matrix proteins excit-ing approach encourage formation around the implant. In cu...

introduction: it has been shown that some primary human tumors and their metastases, including prostate and breast tumors, over-express gastrin-releasing peptide (grp) receptors. bombesin is a neuropeptide with a high affinity for these grp receptors. the purpose of this study was to prepare and evaluate the characteristics of a new freeze-dried kit, [6-hydrazinopyridine-3-carboxylic acid (hyni...

Shiga toxin producing Escherichia coli (STEC) harbouring the stx(2d-activatable) gene and expressing the mucus- and elastase-activatable phenotype have been associated with severe outcomes of human disease. However, there is limited data available on the occurrence of such strains in livestock reservoirs. In this study, we analyzed 11 STEC strains isolated from healthy cattle and sheep at slaug...

Background: Dilated cardiomyopathy (DCM) is revealed with the left ventricular dilatation and systolic dysfunction. This study was performed to determine the level of Calcitonin Gene Related Peptide (CGRP) and Brain Natriuretic Peptide (BNP) in children with dilated cardiomyopathy and controls and comparison of these two biomarkers in patients. Materials and Methods: This case-control study was...

we have employed a peptide-based antibody generation protocol for producing antibody against human nestin. using a 12-mer synthetic peptide from repetitive region of human nestin protein devoid of any n- or o-glyco-sylation sequences, we generated a mouse monoclonal antibody capable of recognizing human, mouse, bovine, and rat nestin. a wide variety of nestin proteins ranging from 140-250 kda w...

Somatostatin analoges labeled with different radionuclides are able used for imaging and treatment of somatostatin receptor positive tumors. In this study Tyr³-Octreotide protected at lysine as an analoge of somatostatin was conjugated with bifunctional chelating agent 6-BOC-hydrazinopyridine-3-carboxylic acid (BOC-HYNIC) and after deprotecting of conjugate, purification was performed wit...

Objective(s): Despite recent advances in treatment modalities, cancer remains a major source of morbidity and mortality throughout the world. Currently, the development of sensitive and specific molecular imaging probes for early diagnosis of cancer is still a problematic challenge. Previous studies have been shown that some of the antimicrobial peptides (AMPs) exhibit...

Background: Antenatal steroid therapy recently has been considered for term and late preterm neonates delivered by Cesarean section (CS), with the aim of preventing adverse respiratory morbidity. The main aim of this study was to investigate the metabolic effects of antenatal dexamethasone on blood glucose (BG), homeostasis, and serum C-peptide level when administered to term fetuses. Methods: ...

Foot and Mouth Disease (FMD) is highly contagious disease among cloven-hoofed animals. FMD virus has structural and non-structural proteins. Vp1 is the most immunogenic structural peptides of FMD virus, applied for major vaccine studies. Objective: Construction of Pet28-VP1 cassette for FMD virus type O/IRN/2010 and expression VP1 peptide as the most immunogenic antigen was the aim of this stud...