In this protocol, we describe an approach in which two-dimensional electrophoresis (2DE)-separated proteins are guanidinated in-gel prior to enzymatic cleavage. In contrast to previously described techniques, this procedure allows the extracted tryptic peptides to be N-terminally sulfonated without any further sample purification. The protocol was applied on a proteomic study of 2DE-separated p...

Union Carbide Witco Chemical Aerosol 22 Aerosol A-102 Aerosol 18 Aerosol OS Cyanamer P-35 Ethofat 242/25 Ethofat C/15 Ethomid HT/60 Ethomeen 18/60 Merpol DA Zelec DX Duponol ST Merpol F Alkanol S Flakes Duponol WA Dry Duponol WA Paste Duponol WAQ Duponol ME Dry FC-134 FC-170 FC-172 LSD-2 LSD-6 Dequest 2000 Dequest 2006 EMA Grade 11 EMA Grade 61 ID-2721 Lomar PW Nopcosant L Tamol SN Tamol 731-25...

A highly sensitive and selective Hg probe 4 based on rhodamine-b was developed and characterized. In consideration of environmental and biological application, we connected a water soluble receptor group (sulfonated β-naphtol) and rhodamine-b together through hydrazine hydrate in high yield. The result turns out that this compound not only exhibits excellent sensitivity and selectivity toward H...

The formation of nonluminescent aggregates of aluminium sulfonated phthalocyanine in complexes with CdSe/ZnS quantum dots causes a decrease of the intracomplex energy transfer efficiency with increasing phthalocyanine concentration. This was confirmed by steady-state absorption and photoluminescent spectroscopy. A corresponding physical model was developed that describes well the experimental d...

a gel composite (based on polyacrylamide and crosslinker cr(iii) acetate) was prepared by solvent method (distillate and water formation) and nanoclay particle (kaolinite). using xrd tests, d001 was evaluated for kaolinite nanoparticles in gel composite. kaolinite modification using dimethyl-sulfoxide led to increase interlamellar spacing from 7.21 to 10.82 o a. based on the results obtained fo...

conclusions our method provided good results and performing evacuation under the guide of bedside ultrasonography is recommended. results we treated 173 patients, including 164 females (94.8%), with the mean (sd) age of 43.75 (10.62) years. according to vas, 95.4% of patients reported complete satisfaction while 2 (1.2%) were unsatisfied with the results. patients reported no complication of th...

drilling fluid is the most important lifeline of the drilling operation, that main task is facilitate the cuttings removal of the drilling. there are varieties of drilling fluids such as sodium bentonite based-drilling fluid is called “mud” and drilling foam or surfactant based-drilling fluid is called “soap”. the present work aims are study on the modified drilling mud properties by using the ...

Re-polymerization reactions are a commonly reported issue on the way to higher recovery of monomers from lignin. The reactivity obtained lignin depolymerization and their contribution re-polymerization in supercritical water (SCW) was investigated. Sulfonated Kraft (SKL) used along with four monomers: vanillin, vanillic acid, vanillyl alcohol acetovanillone. Indulin also employed as reference u...

introduction when a liquid is irradiated with high-intensity and low-frequency ultrasound, acoustic cavitation occurs and there are some methods to determine and quantify this phenomenon. the existing methods for performing these experiments include sonochemiluminescence (scl) and chemical dosimetric methods. the particles in a liquid decrease the ultrasonic intensity threshold needed for cavit...

In this study, ion exchange nanocomposite membranes was prepared by addition of Mg(OH)2 nanoparticles to a blend containing sulfonated polyphenylene oxide and sulfonated polyvinylchloride via a simple casting method. Magnesium hydroxide nanoparticles were synthesized via a facile sono-chemical reaction and were selected as filler additive in fabrication of ion exchange nanocomposite membranes. ...