Forty-eight Holstein cows (16 primiparous) were fed alfalfa silage-based TMR containing 18% CP with 33 or 36% of the CP as undegraded intake protein and with 0 or 2.8% supplemental fat (DM basis). Expeller soybean meal replaced solvent soybean meal to vary undegraded intake protein, and sodium alginate-treated tallow was used as the fat source. A standard diet containing solvent soybean meal wi...

New multicomponent domino reactions of arylglyoxals with pyrazol-5-amines have been established, providing selective access to unprecedented pyrazolo-fused 1,7-naphthyridines, 1,3-diazocanes, and pyrroles (up to 52 examples). The unreported dipyrazolo-fused 1,7-naphthyridines were regioselectively synthesized through a special double [3 + 2 + 1] heteroannulation accompanied by direct C-C format...

Anti-Smaug antibody has been described previously [1]. Immunoprecipitations were performed as previously described [2] with the following modifications: protease inhibitor cocktail consisted of 1 mM 4-(2-aminoethyl)-benzenesulfonyl fluoride, 2 mM benzamidine, 2 ug/mL leupeptin, and 2 ug/mL pepstatin; normal guinea pig serum was used in place of normal rat serum; and Protein A beads were used in...

Identification of Polystyrene (PS)-Binding Sequences Polymer-binding sequences were identified using a phage display library developed from phage type M13. The library displayed random peptide sequences on its pIII coat proteins of the format X6YX6 or X6PX6, where X represents one of the twenty naturally occurring amino acids. For the panning procedure, wells of a native polystyrene plate (CoSt...

Common environmental chemicals, drugs, or physical agents can adversely affect human teeth during their embryonic development and after their eruption into the oral cavity. One of the more common elemental toxicants is lead. Teeth are known to accumulate lead during their development. Both animal and human studies have shown that teeth with high lead levels are generally more susceptible to den...

Ischemia-reperfusion. All experiments were carried out (license A75-19-01, French Department of Agriculture) in accordance with the European Committee’s Council Directive and performed to comply with the ARRIVE (Animal Research: Reporting In Vivo Experiments) guidelines. Ischemia was induced in Wistar P15 rats (Janvier, Le Genest StIsle, France, 32.1±2.1 g, n=72) as previously reported [1]. Bri...

Generation of PIKE knockout mice (PIKE -/-) In order to study the neuronal functions of PIKE-L on GluA2/GRIP1 association in vivo, we generated mice with targeted disruption in the CENTG1 locus via homologous recombination using LoxP/Cre system. We first made a transgenic line with the loxP insertions in intron 2 and 7 of CENTG1 gene (Fig S4A). PIKE flox/+ mice were then bred with transgenic mi...

Following the publication of this article and a corrigendum (1) correcting a microscopy image in Figure 7C, the authors informed the editorial board that multiple histology images in Supplemental Figures 7 and 11 were incorrect. The db/+ vehicle image in Supplemental Figure 7A (representing 30 days of treatment) was taken from the same tissue block as the db/+ vehicle image in Supplemental Figu...