Using commercially available reagents we developed an enzyme-linked immunosorbent assay to measure lactoferrin in normal human tears. Tears obtained from 38 normal human subjects contained a mean lactoferrin content of 2.2 mg/ml (g/l). No differences in the mean level of lactoferrin were found when tears were collected by either Schirmer tear strips or by glass capillaries after short tear gas ...

PURPOSE To document by optical coherence tomography (OCT) the reattachment of the margins of a retinal pigment epithelium (RPE) tear. METHODS Single case report, documented by OCT scans, autofluorescence and fluorescein angiography. RESULTS A 67-year-old male presented with a spontaneous RPE tear due to age-related macular degeneration in his right eye. Three months later we observed that t...

Human tear film fluid offers tremendous potential as a non-invasive diagnostic fluid. However, the use of tear fluid in diagnostic medicine is limited owing to non-standardized collection methodologies and low volume availability. We present a first-reported microfluidic tear fluid biomarker assay which will serve as a foundation for both rapid biomarker validation and point-of-care ocular diag...

OBJECTIVE To evaluate immediate perineal and neonatal morbidity associated with instrumental rotations performed with Thierry's spatulas for the management of persistent posterior occiput (OP) positions. METHODS Retrospective study including all persistent occiput posterior positions with vaginal OP delivery, from August 2006 to September 2007. Occiput anterior deliveries following successful...

This study aimed to analyze the prevalence of skin tears (ST) among hospitalized oncology patients and associated demographic and clinical variables. This is an epidemiological cross-sectional study type, performed at Octavio Frias de Oliveira State of São Paulo Cancer Institute. All adult patients hospitalized from April 10th to 18th 2010 were evaluated by interview and physical examination. C...

To investigate the role of Interleukin-1 (IL-1) in the pathobiology of the cornea, we measured IL-1 beta concentration in tear fluid samples from patients with corneal disease. Twenty patients with unilateral corneal disease were included in the study. Tear fluid samples were collected during the active stages of the disease and following resolution. The fellow (unaffected) eyes served as contr...

PURPOSE The type III secretion system (T3SS) is a significant virulence determinant for Pseudomonas aeruginosa. Using a rodent model, we found that contact lens (CL)-related corneal infections were associated with lens surface biofilms. Here, we studied the impact of human tear fluid on CL-associated biofilm growth and T3SS expression. METHODS P. aeruginosa biofilms were formed on contact len...

This paper describes a technique for simulating the capsulorhexis procedure during cataract surgery on the EYESI system. The continuous curvilinear capsulorhexis technique can be a difficult procedure for beginning ophthalmology surgeons. In the initial phase of tearing the tissue, the tear vector is tangential to the circumference of the tear circle. However, without the proper re-grasping of ...

BACKGROUND The relation between knee meniscal structural damage and cartilage degradation is plausible but not yet clearly proven. OBJECTIVES To quantitate the cartilage volume changes in knee osteoarthritis using magnetic resonance imaging (MRI), and determine whether meniscal alteration predicts cartilage volume loss over time. METHODS 32 patients meeting ACR criteria for symptomatic knee...