in this study we estimated the allele and genotype frequencies of scd1 and dgat1 gene polymorphism. the analysis was conducted on 408 holstein cows from five dairy herds in isfahan province. genotypes were identified using pcr-rflp technique. two genetic variants (a and v) of the scd1 gene were observed in this experiment. the frequency of a-allele ranged from 0.49 to 0.66, while frequency of v...

introduction: endurance training has significant effects on the renewal of heart tissue, including myosin heavy chain (mhc) proteins. on the other side, purine-rich element-binding protein β (purb) decreases the &alphamhc; gene expression. the aim of this study was to determine the impact of exercise endurance training on purb gene expression in the heart of wistar rats. methods: fourteen rats ...

brucellosis, caused by brucella spp., is an important zoonotic disease that causes abortion and infertility in cattle and undulant fever in humans. various studies have examined cell-free native and recombinant proteins as candidate protective antigens in animal models. among brucella immunogenes, antigen based on ribosomal preparation has been widely investigated. in this study, the immunogeni...

methods the current study generated two recombinant lentiviruses, plex-gcn and plex-gcp, bearing noxa and puma, respectively. transduction of both genes to hepatocarcinoma (hepg2) was verified using fluorescent microscopic analysis, western blotting, and quantitative real-time polymerase chain reaction (pcr). to evaluate the potential of noxa and puma to initiate apoptosis, a caspase-9 real-tim...

A multigene analysis of a combined ITS-LSU-SSU-rpb2-tef1 sequence data matrix was applied to infer the phylogenetic position of the genus Thyridaria in the Pleosporales. The generic type of Thyridaria, T. broussonetiae (syn. T. incrustans), is situated in a clade currently named Roussoellaceae, which becomes a synonym of Thyridariaceae. However, Thyridaria rubronotata does not belong to this cl...

During a survey of cold-adapted fungi in alpine glaciers on the Qinghai-Tibet Plateau, 1 428 fungal isolates were obtained of which 150 species were preliminary identified. Phoma sclerotioides and Pseudogymnoascus pannorum were the most dominant species. Psychrotolerant species in Helotiales (Leotiomycetes, Ascomycota) were studied in more detail as they represented the most commonly encountere...

tRNAs in yeast and vertebrate cells move bidirectionally and reversibly between the nucleus and the cytoplasm. We investigated roles of members of the beta-importin family in tRNA subcellular dynamics. Retrograde import of tRNA into the nucleus is dependent, directly or indirectly, upon Mtr10. tRNA nuclear export utilizes at least two members of the beta-importin family. The beta-importins invo...

Rho GTPases are molecular switches that modulate a variety of cellular processes, most notably those involving actin dynamics. We have previously shown that yeast vacuolar membrane fusion requires re-organization of actin filaments mediated by two Rho GTPases, Rho1p and Cdc42p. Cdc42p initiates actin polymerization to facilitate membrane tethering; Rho1p has a role in the late stages of vacuola...

Species of Nigrospora commonly occur as plant pathogens, endophytes or saprobes, and have been shown to be extremely interesting for the discovery of novel metabolites. The familial placement, as well as phylogenetic relationships among Nigrospora species remain ambiguous. In this study, Nigrospora (= Khusia) is confirmed as a monophyletic genus belonging to Apiosporaceae (Xylariales), based on...

The order Phyllachorales (Pezizomycotina, Ascomycota) is a group of biotrophic, obligate plant parasitic fungi with a tropical distribution and high host specificity. Traditionally two families are recognised within this order: Phyllachoraceae and Phaeochoraceae, based mostly on morphological and host characteristics. Currently, the position of the order within the class Sordariomycetes is inco...