Glutamine phosphoribosylpyrophosphate amidotransferase from Bacillus subtilis is synthesized as an inactive precursor that requires two maturation steps: incorporation of a [4Fe-4S] center and cleavage of an 11residue NH2-terminal propeptide. Overproduction from a multicopy plasmid in Escherichia coli leads to the formation of soluble proenzyme and mature enzyme forms as well as a small fractio...

We aimed to evaluate the potential of growing rats as a model of osteoporosis induced by short term hypercholesterolemia. Twelve growing female Sprague-Dawley rats were randomly allocated into two groups. Control rats received standard diet while rats in group 2 were fed with diet contained 20% sunflower oil, 2% cholesterol and 0.5% cholic acid for 2 weeks and then a diet contained 10% sunflowe...

The body wall of hydra (a member of the phylum Cnidaria) is structurally reduced to an epithelial bilayer with an intervening extracellular matrix (ECM). Previous studies have established that cell-ECM interactions are important for morphogenesis and cell differentiation in this simple metazoan. The ECM of hydra is particularly interesting because it represents a primordial form of matrix. Desp...

Study purpose. Exercise training plays an important role in increasing bone mass. Nevertheless, the osteogenic effects of exercise using various bench surfaces are unknown. Therefore, this study aimed to compare with a soft surface and aerobic both sides utilized (BOSU) ball those step (STEP). Materials methods. Fifty-two sedentary female participants aged 30–45 years were recruited randomly di...

The premature GDF/PDF/MIC-1 protein consists of 308 amino acids that contain a 29 amino acid signal peptide, a 167 amino acid propeptide, and a 112 amino acid mature protein. The mature protein is secreted as a homodimer linked by disulfide bonds and is released from the propeptide following intracellular cleavage at RXXR furine-like cleavage site. The mature peptide of GDF-1/MIC-1 contains two...

Background After myocardial infarction (MI) complicated by heart failure (HF), eplerenone reduced serum concentrations of amino-terminal propeptide type III collagen (PIIINP) and carboxy-terminal I (PICP). Determining a subgroup who are more prone to decrease their content respond better the antifibrotic effects mineralocorticoid receptor antagonists (MRA) may be relevant for personalized treat...

alpha-1-Antitrypsin is found in hepatocytes as a high-mannose glycoprotein (Mr 49 000), extracellularly as a complex-type glycoprotein (Mr 54 000). Deglycosylation of both forms with peptide: N-glycosidase led to proteins of identical app. Mr (41 000). The sequence of 26 N-terminal amino acids of rat alpha 1-antitrypsin was determined. A high content of polar amino acids was found. The partiall...