We have previously isolated and sequenced a portion of the structural gene for tetanus toxin from C.tetani CN3911 (1). Overlapping clones encoding the entire gene have now been isolated and the full sequence is reported below. Our designation of the start of translation is based on amino terminal analysis (2) and partial ami no acid sequence of the toxin (3). The methionine residue before the p...

1. Both diphtheria and tetanus toxin and their antitoxins arc electro-positive, that is, they pass to the cathode under the influence of an electric current. 2. The character of the charge is not altered by a change in the reaction of the solvent. 3. The combination of toxin and antitoxin would seem to represent not a true chemical reaction but the adsorption of one colloid by another.

The Rockefeller University Press $30.00 J. Cell Biol. Vol. 205 No. 2 217–232 www.jcb.org/cgi/doi/10.1083/jcb.201311003 JCB 217 Correspondence to Stephanie L. Gupton: [email protected] Abbreviations used in this paper: ANOVA, analysis of variance; BoNTA, botulinum toxin A; CC, coiled coil; CMV, cytomegalovirus; DCC, deleted in colorectal cancer; DIC, differential interference contrast; LD, l...

GAL4-driven targeted expression of tetanus toxin light chain (UAS-TeTxLC) in a subset of chemosensory neurons of the larval antennomaxillary complex (AMC) and pharynx causes abnormal chemosensory behavior in Drosophila melanogaster. Consistent with strongest staining in the dorsal organ (DO), the presumed olfactory organ of the AMC, tetanus toxin-expressing larvae subjected to an olfactory pref...

We show that mouse neural crest cells cultured in a serum-deprived chemically defined medium on appropriate culture substrata can be induced to express a neuronal phenotype. The uncommitted neural crest cells express a mesenchymal intermediate filament protein such as vimentin, but not the usual neuronal markers such as receptor sites for tetanus toxin or neurofilaments. In the chemically defin...