A shortened 8-mer ssDNA aptamer was successfully truncated for four different tetracyclines with high affinity. The ultrasensitive colorimetric detection of oxytetracycline using this shortened aptamer was possible, which was about 500-fold enhanced compared to that obtained using the original 76-mer aptamer.

The in vitro activities of GAR-936, the 9-t-butylglycylamido derivative of minocycline, were compared with those of doxycycline, minocycline, and tetracycline against 527 gram-positive clinical isolates. GAR-936 inhibited all strains, including those resistant to other tetracyclines, at concentrations of </=2 microg/ml, except two strains of JK diphtheroids for which the MIC was 4 microg/ml.

The concentrations of doxycycline and 4-de-dimethylaminotetracycline required to inhibit 50% of collagenase activity were found to be 15 to 30 microM for human neutrophil and gingival crevicular fluid collagenases. Fibroblast collagenase was relatively resistant to inhibition by tetracyclines; the 50% inhibitory concentrations of doxycycline and 4-de-dimethylaminotetracycline were 280 and 510 m...

MICs of many antibiotics for Francisella tularensis are low in axenic medium, whereas only aminoglycosides, tetracyclines, and fluoroquinolones are useful in treating tularemic patients. In an in vitro cell system, only these antibiotics, rifampin, and telithromycin were bactericidal against intracellular F. tularensis. These results correlate better with clinical data than MIC data do.

1. Abstract 2. Introduction 3. Actinomycetes 4. Antibiotic discovery-The odyssey begins 5. The early years 6. The golden period of antibiotics 7. Significant actinomycete antibioticspast to present 7.1 Streptomycin 7.2 Tetracyclines 7.3 Chloramphenicol 7.4 Neomycin 7.5 Erythromycin 7.6 Vancomycin 7.7 Kanamycin 7.8 Rifamycin 7.9 Gentamicin 7.10 Daptomycin 8. Recent advances 8.1 Platensimycin 8.2...

Tetracyclines are broad spectrum antibiotic, belong to the family of polyketide antibiotic ,produced by Streptomyces aureofaciens of actinobacteria. The organism Streptomyces aureofaciens 325 was obtained MTCC and cultured on the starch casein media. Screening the organism for the production was confirmed by antibiogram test. The production media was optimized by using carbon sources, and nitro...