Shim, J.-S., Farrand, S. K., and Kerr, A. 1987. Biological control of crown gall: Construction and testing of new biocontrol agents. Phytopathology 77:463-466. Transfer deficient (Tra-) Tn5 insertion mutants of the agrocin 84 plasmid strain K84 in controlling crown gall. Strains of Agrobacterium radiobacter pAgK84 were transformed into two chromosomal backgrounds and the which contained a high ...

Historically, nmuch of the research in plant tumor physiology and growth has utilized cultures derived from bacterial infection and wounding. Observation of these systems has demonstrated that the transformation of a nornmal cell to a tumor cell in the crown-gall disease involves progressive activation of certain biosynthetic systems. According to Braun and Wood (3) transformed cells become aut...

Anguina pacificae is distributed along a narrow strip on the Pacific coast of Northern California where it forms galls on the shoots of Poa annua and causes significant damage to golf course greens. Methods were developed for the continuous propagation of A. pacificae on P. annua in growth chambers, and they were used to examine the life cycle and host-parasite relationships of the nematode. At...

The organization of gall on the ovary ofSesamum indicum L induced by the gall midge, Asphondylia sesami Felt, not only affects biochemical equilibrium of the host but also causes disturbed vegetative growth and reduced seed-setting. The toxicity created by the gall maker induces the re-orientation of vasculature Cecidogenetic stimuli lead to excessive hypertrophy and hyperplasy forming the gall...

The effect of high temperature treatments on the early stages of crown gall tumorigenesis in sunflowers was investigated. Treatments of 32 C initiated at various times during the first ten days after infection had a similar effect on tumors induced by Agrobacterium tumefaciens strains B(6) and C58. Tumor growth was sensitive to 32 C until 60 to 72 hours after infection and was stimulated by 32 ...

The respiratory processes of malignant tissues of animals and human beings have long been considered to differ in important respects from those of nonmalignant tissues of the same creatures. This difference expresses itself in several ways, especially in a low R.Q. (0.8 or less) and a decided anaerobic tendency to accumulate acid products, lactic acid in particular. It seems possible, indeed, i...

The endogenous levels of auxin and cytokinin in teratoma and unorganized tobacco (Nicotiana tabacum L. var Wisconsin #38) crown gall tumor tissues were determined. Teratoma tissues contain levels of auxin and cytokinin favorable for shoot formation, whereas unorganized tumors contain levels of auxin that suppress shoot formation. This conclusion is based upon the observation that when levels of...

A cloned tobacco crown gall tumor, 1595501, incited by A. tumefaciens strain 15955 was studied. Molecular analysis of the organization of the T-DNA by means of Southern transfer and hybridization techniques indicated that the 1595501 tumor has about 10 copies of TL DNA, five of which are complete TL DNA, whereas most octopine tumors have only one to two copies of complete TL DNA. Hybridization ...

Experiments using different species of the plant Nicotiana and strains of the bacterium Agrobacterium tumefaciens showed that teratoma formation from crown galls was dependent on the combination of bacterial Ti plasmid and host plant used.