In vivo homologous recombination holds the potential for optimal molecular cloning, however, current strategies require specialised bacterial strains or laborious protocols. Here, we exploit a recA-independent recombination pathway, present in widespread laboratory E.coli strains, to develop IVA (In Vivo Assembly) cloning. This system eliminates the need for enzymatic assembly and reduces all m...

Due to the advantages of high information densities and longevity, DNA storage systems have begun attract a lot attention. However, common obstacles are caused by insertion, deletion, substitution errors occurring in synthesis sequencing. In this paper, we first explain method convert binary data into general maximum run-length $r$ sequences with specific length construction, which can be used ...

Building industry as one of the greatest industries in Iran comprises a significant share of waste generation in the country. Since a large quantity of the generated construction waste is buried due to the lack of a recycling protocol, it leads to an undesired waste of resources and causes severe environmental issues. This paper provides a study on the problem of material loss/construction wast...

effect of lidocaine gel on pain from copper iud insertion: a randomized dobell blind controlled trial     mohammad alizadeh s [1] , kazami f [2] , farshbafkhalili a [3] , saidi s [4]     received: 19 feb, 2012 accepted: 20may, 2012     abstract   background & aims : the copper bearing intrauterine device (iud) is a long-term, reversible contraception method that is as effective as tubal sterili...

Aims. The aim of the study was to compare endotracheal intubation with i-gel and LMA (laryngeal mask airway) supreme supraglottic airway devices in orbital osteosynthesis surgery. Methods. 134 patients undergoing orbital plasty after fracture were enrolled in our study and were randomized into three groups: 43 in the endotracheal intubation group, 42 in the LMA-Supreme group, and 49 patients w...

A more flexible and higher-yielding in vitro DNA mismatch repair (MMR) substrate construction method, which was developed initially by Wang and Hays, is described for the construction of a nucleotide-based chemical mismatch (G/IU) and a G/T mismatch. Our modifications use the combination of two endonuclease enzymes (NheI and BciVI) and two new redesigned plasmids (pWDAH1A and pWDSH1B). In our m...

We explore some generalizations of fullerenes Fv (simple polyhedra with v vertices and only 5and -gonal faces) seen as (d−1)dimensional simple manifolds (preferably, spherical or polytopal) with only 5and 6-gonal 2-faces. First, finite and planar (infinite) 3-fullerenes are described. Three infinite families of spherical 4fullerenes are presented in Sections 4-6. The Construction A gives 4-poly...