In this study, we developed a novel method for fabricating microwell arrays constructed from alginate gels, and the alginate gel microwells were used for three-dimensional (3D) cell culture. The alginate gel microwells were fabricated on a patterned ITO electrode using alginate gel electrodeposition. Embryonic stem (ES) cells or hepatocellular carcinoma cells (HepG2) were cultured in the algina...

Stem cell transplantation therapy using mesenchymal stem cells (MSCs) is considered a useful strategy. Although MSCs are commonly isolated by exploiting their plastic adherence, several studies have suggested that there are other populations of stem and/or osteoprogenitor cells that are removed from primary culture during media replacement. Therefore, we developed a three-dimensional (3D) cult...

In vitro cell-based models of lung cancer are frequently employed to study invasion and the mechanisms behind metastasis. However, these models often study only one cell type with two-dimensional (2D) monolayer cell cultures, which do not accurately reflect the complexity of inflammation in vivo. Here, a three-dimensional (3D) cell co-culture collagen gel model was employed, containing human lu...

Basic and translational research on lung biology has discovered multiple progenitor cell types, specialized or facultative, responsible for turnover, renewal, and repair. Isolation of populations of resident lung progenitor cells (LPCs) has been described by multiple protocols, and some have been successfully applied to healthy human lung tissue. We aimed at understanding how different cell cul...

Most of river engineering problems are complicated and need to be analyzed by three dimensional models of flow and sediment transport. Considering the fact that computers and numerical methods with great ability of flow and sediment transport simulation are developing quickly, using numerical modeling by engineers is inevitable. Nevertheless, before application, numerical models need to be veri...

in this paper, three dimensional (3d) static and dynamic analysis of thick plates based on the meshless local petrov-galerkin (mlpg) is presented. using the kinematics of a three-dimensional continuum, the local weak form of the equilibrium equations is derived. a weak formulation for the set of governing equations is transformed into local integral equations on local sub-domains by using a uni...

This study was conducted to determine the use of three-dimensional ultrasonography (3DU) in ocular imaging of the dog and measuring of its optical axis. 12 healthy mixed-breed dogs including 6 males and 6 females were studied. 3DU of the eyes were done using a 5–12 MHz linear trapezoid transducer. 3DU of the eyes were evaluated and the normal optical long axis through a line between the cornea ...

A method for optimizing MR imaging pulse sequence parameters in a statistical framework is presented. Parameters are defined to be optimal when the resulting scalar images yield optimal image segmentations using Bayesian pixel classification. Thus, Bayes risk is used as the objective function to minimize. Approximations are made to give a tractable solution in a four-step procedure. A sample ca...

Several optimal three-dimensional orbital transfer problems are solved for thrust-limited spacecrafts using collocation and nonlinear programming techniques. The solutions for full nonlinear equations of motion are obtained where the integrals of the free Keplerian motion in three dimensions are utilized for coasting arcs. In order to limit the solution space, interior-point constraints are use...