In this study, we present confocal Raman microscopy for chemically selective analysis of a human buccal epithelial cell layer with a focus on label-free visualization of particle uptake into the cells. We demonstrate the suitability and benefit of this analytical technique in comparison to confocal fluorescence microscopy for three dimensional imaging of in vitro cell models.

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Live imaging in cell biology requires three-dimensional data acquisition with the best resolution and signal-to-noise ratio possible. Depth aberrations are a major source of image degradation in three-dimensional microscopy, causing a significant loss of resolution and intensity deep into the sample. These aberrations occur because of the mismatch between the sample refractive index and the imm...

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Diffuse optical tomographic imaging is known to be an ill-posed problem, and a penalty/regularization term is used in image reconstruction (inverse problem) to overcome this limitation. Two schemes that are prevalent are spatially varying (exponential) and constant (standard) regularizations/penalties. A scheme that is also spatially varying but uses the model information is introduced based on...

Tomographic diffractive microscopy is a three-dimensional imaging technique that reconstructs the permittivity map of the probed sample from its scattered field, measured both in phase and in amplitude. Here, we detail how polarization-resolved measurements permit us to significantly improve the accuracy and the resolution of the reconstructions, compared to the conventional scalar treatments u...

Electrophysiological mapping and ablation techniques are increasingly used to diagnose and treat many types of supraventricular and ventricular tachycardias. These procedures require an intimate knowledge of intracardiac anatomy and their use has led to a renewed interest in visualization of specific structures. This has required collaborative efforts from imaging as well as electrophysiology e...

This review article aims to describe and discuss the imaging techniques most commonly used in medicine and dentistry to obtain three-dimensional images of the craniofacial complex. Three-dimensional imaging techniques provide extensive possibilities for the detailed and precise analysis of the whole craniofacial complex, for virtual (on-screen) simulation and real simulation of orthognathic sur...

Automated human face recognition is required in numerous applications. While considerable progress has been made in color/two dimensional (2D) face recognition, three dimensional (3D) face recognition technology is much less developed. 3D face recognition approaches based on the appearance of range images and geometric properties of the facial surface have been proposed. Methods that combine 2D...

T h i s paper describes C A B O T , a case-based syst e m t h a t is able to ad jus t i ts re t r ieva l and adapt a t i o n met r ics , in a d d i t i o n to s to r ing cases. I t has been app l ied to the game of OTHELLO. Exper iments show t h a t C A B O T saves a b o u t ha l f as many cases as s imi la r systems t h a t do no t ad jus t the i r re t r ieva l and a d a p t a t i o n mechani...