نتایج جستجو برای: time nasba
تعداد نتایج: 1891542 فیلتر نتایج به سال:
مقدمه: با توجه به محدودیت های برخی از روش های سرولوژیک در تشخیص افراد دارای عفونت با ویروس های hiv-1 و hcv، استفاده از روش های تشخیصی ملکولی در شناسایی این دو عامل اهمیت بسیار زیادی دارد. با این حال اشکال عمده ی روش های ملکولی هزینه بر بودن آن ها و نیاز به وجود دستگاه ترموسایکلرکه گران قیمت است. روش ها: این پژوهش به راه اندازی و به کارگیری یک روش nasba (nucleic acid sequence based amplification...
Molecular methods have been proposed as highly sensitive tools for the detection of Leishmania parasites in visceral leishmaniasis (VL) patients. Here, we evaluate the diagnostic accuracy of these tools in a meta-analysis of the published literature. The selection criteria were original studies that evaluate the sensitivities and specificities of molecular tests for diagnosis of VL, adequate cl...
Listeria monocytogenes is an important food-borne pathogen and is widely tested for in food, environmental and clinical samples. Identification traditionally involved culture methods based on selective enrichment and plating followed by the characterization of Listeria spp. based on colony morphology, sugar fermentation and haemolytic properties. These methods are the gold standard; but they ar...
The paper presents the development of a "proof-of-principle" hands-free and self-contained diagnostic platform for detection of human papillomavirus (HPV) E6/E7 mRNA in clinical specimens. The automated platform performs chip-based sample preconcentration, nucleic acid extraction, amplification, and real-time fluorescent detection with minimal user interfacing. It consists of two modular protot...
The prognostic significance of human papillomavirus (HPV) DNA and E6/E7 mRNA, the presence of specific types, and the physical state of HPV DNA, were studied in 202 cervical squamous cell carcinomas. Absence or non-detectable levels of high-risk (types 16, 18, 31, 33, 35, 45, 52 and 58) E6/E7 mRNA, using the real-time nucleic acid sequence based amplification (NASBA) assay, and absence of HPV h...
Dengue fever is considered the most important arthropod-borne viral diseases in terms of morbidity and mortality. An accurate and efficient diagnosis of dengue plays an important role in case confirmation. The virus may be isolated during the viremic phase (within day 5 of illness), from serum, plasma and peripheral blood mononuclear cells. Enzyme linked immunoassay (ELISA) has demonstrated the...
background and objectives: rapid, accurate and cost effective diagnosis of infectious and non infectious diseases is an essential step for treatment process. nowadays, in line with scientific progression in molecular biology, genetics and biochemistry which are based on biotechnology and genetic engineering aspects, new branch of medicine entitled molecular medicine is being derived. it can be ...
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