A lthough heart disease is the principal cause of death in many countries and its diagnosis is of major importance, methods for direlct visualization of the myocardium in intact subjects are few. The roentgenogram of the heart shows the myocardium plus other tissues, especially the blood contained in the cardiac cavities, as a single shadow. Various techniques show cavities and vessels rather t...

hydrogen peroxide is an important mild oxidant which is widely used in various applications including remediation of waste waters and drinking water resources. the safe level of h2o2 residue has to be controlled through accurate and precise measurement methods. a facile spectrophotometric method for the determination of hydrogen peroxide is based on the reaction of iodine solution with toluidin...

Tissue-engineering is considered a promising avenue for developing human articular cartilage implants that can be employed for resurfacing damaged cartilage in the early stages of osteoarthritis. In the present study, human cartilage-constructs were produced from human osteoarthritic chondrocytes maintained on a scaffold of HYAFFR-11 in perfusion mini-bioreactors or after implantation and recov...

Genes controlling carotenoid synthesis were cloned from Erwinia herbicola and expressed in an Escherichia coli strain. Carotenoids protect against high fluences of near-UV (NUV; 320 to 400 nm) but not against far-UV (200-300 nm). Protection of E. coli cells was not observed following treatment with either psoralen or 8-methoxypsoralen plus NUV. However, significant protection of cells producing...

A serosurvey for Pneumocystis carinii infection of laboratory-bred and wild-born monkeys was made by the indirect immunofluorescence method. The antibody titers of wild cynomolgus (Macaca fascicularis) and Japanese monkeys (Macaca fuscata fuscata) to P. carinii (Pc) were higher than those of laboratory-bred cynomolgus monkeys; 54.9% of the wild cynomolgus and 40% of Japanese monkeys had antibod...

Immunodeficient animals are in demand in current biomedical research, and they contribute to medical progress. In Pneumocystis infections, a specific histological diagnostic tool may be immunochemistry (IC). However, it was recently reported that the antibody (3F6) was not suitable for detecting Pneumocystis in rats. We purchased another antibody [PNC007] from a commercial source for IC. We cou...

To optimize the function of implants the formation of surrounding connective tissue should be adapted in dependence to the mechanical conditions. Therefore, with nanostructured silica coated Bioverit® II implants were only partly reacted with recombinant BMP-2. The histology was compared 28, 84 and 301 days after implantation in the rabbit middle ear and subcutis, respectively. The whole tissue...

A protocol for the bacteriophage amplification technique was developed for quantitative detection of viable Listeria monocytogenes cells using the A511 listeriophage with plaque formation as the end-point assay. Laser and toluidine blue O (TBO) were employed as selective virucidal treatment for destruction of exogenous bacteriophage. Laser and TBO can bring a total reduction in titer phage (ca....