entire lesion was fixed in Bouin's fluid. The specimen was sectioned and stained with hematoxylin and eosin, Bodian's silver impregnation, phosphotungstic acid-hematoxylin, Masson's trichrome, PAS2 -Alcian blue, toluidine blue at pH 2 and pH 4, and Gomori's reticulum stain. This lesion is registered as Specimen RTLA 272 in the Registry of Tumors in Lower Animals, Museum of Natural History, Smit...

The use of periodic acid oxidation followed by treatment with Schiff's reagent as a method for staining polysaccharides was first reported briefly by McManus (1946) and later in some detail by Hotchkiss (1948). Hotchkiss did not find the procedure particularly satisfactory for bacteria, but considered that it worked very well with many other polysaccharide materials. Despite the poor results re...

AIM To compare the effectiveness of self-examination, clinical examination and screening methods using 1% toluidine blue and Lugol's iodine in estimating the prevalence of lesions at risk of malignancy and oral malignant disease amongst the male inmates of Yerwada Central Jail, Pune. MATERIAL AND METHODS Study was carried out on male inmates in two phases. In the first phase self-examination ...

The objective of this study was to investigate the utility of toluidine blue and brush cytology in patients with clinically detected oral mucosal lesions. Clinical examination of 35 patients was completed before toluidine blue application, oral brush cytology and scalpel biopsy. Lesions were photographed before and after stain application; followed by brush cytology. All findings were compared ...

Introduction: Verrucous carcinoma is one of squamous cell in the oral cavity which can caused by smoking, alcohol consumption, HPV etc. mimick as pre cancer lesion such leukoplakia, so that early detection mandatory. Vital staining be done toluidine blue, but it reported toxic organ. Methylene blue had similar chemical structure and exhibited physicochemical properties to blue.The aim this case...

Concurrent acariosis due to sarcoptes scabiei var cuniculi (sarcoptes scabiei) and notoedres cati var cuniculi (notoedres cuniculi) was observed in Angora and New Zealand white rabbits belonging to the University rabbitry at Srinagar in Jammu and Kashmir (India). Clinically, the affected rabbits revealed scales, lichenification, scab formation, alopecia and pruritis initially on the face near t...

BACKGROUND It is estimated that about 50% of causes of recurrent pregnancy loss (RPL) cases remain unknown. Sperm factors are suggested to have probable role in cases with RPL. OBJECTIVE The goal was to determine the possible relationship between semen bacterial contaminations with unexplained RPL. Also, the correlation between number of bacterial colony and sperm chromatin condensation was e...

Human adipose derived stem cells (hASCs) can be easily isolated and their plasticity has been well characterized. Several TGF-β superfamily ligands can direct hASCs towards chondrocytes. However, these ligands are difficult to purify and expensive. We have developed a library of Activin/BMP2 chimeric ligands (AB2 ligands) by systematically mixing their sequence segments and have tested their ch...

BACKGROUND To evaluate the effectiveness of Microlux/DL with and without toluidine blue in screening of potentially malignant and malignant oral lesions. MATERIALS AND METHODS In this diagnostic clinical trial clinical examination was carried out by two teams: 1) two oral medicine consultants, and 2) two general dentists. Participants were randomly and blindly allocated for each examining tea...