The trabecular meshwork (TM) is the main outflow pathway in the mammalian eye. Oxidative damage to TM cells has been suggested to be an important cause of impairment of TM functions, leading to deficient drainage of aqueous humor, with deleterious consequences to the eye. Transferrin, a metalloprotein involved in iron transport, has been characterized as an intrinsic eye protein. Since transfer...

BACKGROUND Isoforms of transferrin interfere with measurement of carbohydrate-deficient transferrin (CDT) as a marker of heavy alcohol consumption. We evaluated the rate of inaccurate CDT results by immunoassays. METHODS We studied 2360 consecutive sera (1614 individuals) submitted for CDT assay without clinical information as well as samples from 1 patient with a congenital disorder of glyco...

carbohydrate-deficient glycoprotein syndrome. Electrophoresis 1997;18: 1819–26. 12. Tagliaro F, Crivellente F, Manetto G, Puppi I, Deyl Z, Marigo M. Optimised determination of carbohydrate deficient transferrin isoforms (CDT) in serum by capillary zone electrophoresis. Electrophoresis 1998;19:3033–9. 13. Crivellente F, Fracasso G, Valentini R, Manetto G, Riviera AP, Tagliaro F. Improved method ...

DeltaFerrin(TM), a yeast-derived recombinant human transferrin produced by Delta Biotechnology Ltd. (Nottingham UK), was found to be a suitable replacement for holo human transferrin in serum-free culture media of the MDCK cell line (chosen because of its transferrin dependence) in short-term screening assays. Long-term subculture was achieved with DeltaFerrin(TM) supporting growth equivalent t...

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Background. Transferrin is a negative acute phase protein, which decreases during inflammation and infection. The aim of the present investigation was to evaluate changes in the transferrin serum levels subsequent to non-surgical treatment of chronic periodontal disease. Methods. Twenty patients with chronic periodontitis and 20 systemically healthy subjects without periodontal disease, who had...

The human colon, intratumoral subpopulations HCT 116 and HCT 116a were established in chemically defined medium supplemented with transferrin, insulin, epidermal growth factor (EGF), triiodothyronine, hydrocortisone, and sodium selenite. The responsiveness of the adapted cell lines to these growth factors was compared in anchorage-dependent and -independent assays. HCT 116 cells maintained in s...

Pseudomonas aeruginosa placed across a dialysis membrane from [55Fe]transferrin caused the mobilization of the iron from the transferrin side to the bacterial or dialysate side of the membrane. Although the bacteria were capable of obtaining iron from transferrin for growth, the siderophores of P. aeruginosa failed to convert iron bound to transferrin into dialyzable, low-molecular-weight chela...

The present studies were performed to identify reticulocyte membrane receptors for transferrin. Rat reticulocytes were labeled in vitro with 1251 via lactoperoxdose; cell ghosts were then prepared and solubilized with Triton X-100. Binding of soluble membrane1251 components to transferrin conjugated to Sepharose (Seph. tfn) was three times greater than to albumin conjugated to Sepharose (Sephal...

The present studies were performed to identify reticulocyte membrane receptors for transferrin. Rat reticulocytes were labeled in vitro with 1251 via lactoperoxdose; cell ghosts were then prepared and solubilized with Triton X-100. Binding of soluble membrane1251 components to transferrin conjugated to Sepharose (Seph. tfn) was three times greater than to albumin conjugated to Sepharose (Sephal...