Tris-1,10-phenanthroline manganese(II) complex was synthesized by microwave irradiation. The synthesized complex was characterized by UV-visible and FT-IR spectroscopy. Its electrochemical behaviour was examined by cyclic voltammetry (CV). The tris-1,10-phenanthroline manganese(II) complex was used for the electrochemical sensing of catechol. The manganese ion gets reduced as the oxidation of c...

The effect of Tris, Glycine and Tricine on alkaline phosphatase activity was tested at a pH of 8.6, 8.85 and 9.1, using pNPP as substrate. Alkaline phosphatase activity was measured by monitoring the increase in NP absorbance at 420nm using a spectrophotometer. Alkaline phosphatase in Tris buffer at pH 9.1 was found to have the highest activity. MichaelisMenten enzyme kinetic analysis revealed ...

The medium composition was as follows : 0.15 M-sucrose, 30rn~-KCl, 1OmM-choline chloride, 10mM-succinate/Tris, lOmM-Tris/Mops, 3mM-P,/Tris, 2pM-rotenone, 0.1 mMEGTA and 2mg of rat-liver mitochondria/ml; pH7.4. Temperature 25°C. Respiration was stimulated by 0.5 m ~ ADP ( 0 ) in the presence of 1 mM-EDTA, by 20pmol of FCCP/mg (0 ) or by 6Opmol of FCCP/mg (0). When the respiration was stimulated ...

The objective of this study was to evaluate quality of frozen-thawed bull semen processed with extenders containing vitamin C and E as antioxidants. Pooled semen's were collected from 5 bulls and diluted to a concentration of 30 × 106 sperm/mL with citrate –egg yolk (CEY) or Tris – egg yolk (TEY) extenders. The diluted semen was divided to 5 aliquots including control and 4 experimental groups....

the objective of this study was to evaluate quality of frozen-thawed bull semen processed with extenders containing vitamin c and e as antioxidants. pooled semen's were collected from 5 bulls and diluted to a concentration of 30 × 106 sperm/ml with citrate –egg yolk (cey) or tris – egg yolk (tey) extenders. the diluted semen was divided to 5 aliquots including control and 4 experimental groups....

abstract plant nonspecific lipid transfer proteins (nsltps) are divided into nsltp1 and nsltp2. the existence of an internal hydrophobic cavity, is a typical characteristic of nsltps that serves as the binding site for lipid substrates. in this communication a simple, rapid and low-cost alternative method was developed for purification of nsltp2 from rice paddy. after extracting, final supernat...

abstract digestive enzyme inhibitors are proteinacious or nonproteinacious compounds which reduce an enzyme activity through attaching to its active site and/or its substrate. nowadays, plant enzyme inhibitors are of great importance because 1) these have considerable effects on insect digestive enzymes and as a result on their development; and 2) the transgenic plants expressing them are safe....

Two potentially heptadentate (N4O3) tripodal Schiff-base ligands: tris(3(salicylideneimino)propyl)amine (H3L1) and tris(3-(4'-hydroxysalicylideneimino)propyl)amine (H3L2) have been prepared and characterized by various spectroscopic methods (IR, FAB-MS, NMR). They are derived from the condensation reactions of tris(3-aminopropyl)amine (tpt), with 3 equivalents of either salicylaldehyde or the r...

We report a generic approach for targeting proteins into micropatterns by in situ laser lithography. To this end, we have designed a photocleavable oligohistidine peptide for caging tris(nitrilo triacetic acid) (tris-NTA) groups on surfaces by multivalent interactions. Local photofragmentation of the peptide by UV illumination through a photomask or by a confocal laser beam uncages tris-NTA, th...

known to have a coordination number of seven. Nearly all of them have the basic formulation [Ln(bidentate)3(unidentate)]. On the contrary, structurally characterized examples of a heptadentate ligand coordinating one metal ion are rare. Tris(2-aminoethyl)amine (tren) condenses with salicylaldehyde or its substituted analogs in a 1:3 molar ratio to form Schiff-bases which are potentially tribasi...