We study theoretically the capturing of paramagnetic beads by a magnetic field gradient in a microfluidic channel treating the beads as a continuum. Bead motion is affected by both fluidic and magnetic forces. The transfer of momentum from beads to the fluid creates an effective bead-bead interaction that greatly aids capturing. We demonstrate that for a given inlet flow speed a critical densit...

Electron microscopic studies confirm and extend the conclusions derived previously from a quantitative biochemical study of the phagocytosis of polystyrene and polyvinyltoluene latex beads by Acanthamoeba (1). Latex beads 1.305, 1.90, and 2.68 micro in diameter are ingested individually, with each bead tightly surrounded by a membrane derived from the plasma membrane. Latex beads 0.557, 0.264, ...

We use Wang-Landau and replica exchange techniques to study the effect of an increasing stiffness on the formation of secondary structures in protein-like systems. Two possible models are considered. In both models, a polymer chain is formed by tethered beads where non-consecutive backbone beads attract each other via a square-well potential representing the tendency of the chain to fold. In ad...

Probiotics play an important role in human gut health. However, it remains a challenge to maintain the viability of probiotics throughout gastrointestinal transit. Hence, this study aimed microencapsulate Bifidobacterium lactis Bi-07 with galactooligosaccharides (GOS) using co-extrusion technique. Optimization calcium chloride (1.0% w/v 3.0% w/v) and GOS concentration 5.0% were performed encaps...

By sequentially pushing micro-beads towards and away from a sensing surface, we show that ultrasonic radiation forces can be used to enhance the interaction between a functionalised glass surface and polystyrene micro-beads, and identify those that bind to the surface by illuminating bound beads using an evanescent field generated by guided light. The movement towards and immobilisation of stre...

| We consider the following problem: Let r be a n-bead rosary with m white beads and n ? m black beads. Let t be an integer, t n. Denote by MC t (r) the number of pairs, of monochromatic beads which are within distance t apart, in the rosary r. What is the minimum value of MC t (), when the minimum is taken over all n-bead rosaries which consists of m white beads and n ? m black beads? We prove...

We describe a solid-phase assay that has permitted further analysis of zona pellucida glycoprotein, ZP3, as sperm receptor and acrosome reaction-inducer during fertilization in mice. The assay employs silica beads that contain epoxy groups to which purified, mouse oocyte ZP3 is covalently linked (ZP3-beads). ZP3-beads were characterized, e.g. by whole-mount autoradiography and flow cytofluorome...

Cored beads (also known as pellicular, superficially porous, and fused-cored beads or particles) offer advantages over fully porous beads in reduced diffusional mass transfer resistances in particle macropores and separation times in liquid chromatography (LC). They are also used to regulate bead densities. The core of a bead has a relatively small volume and yet presents a large linear distanc...

A high-speed and hardware-only algorithm using a center of mass method has been proposed for single-detector fluorescence lifetime sensing applications. This algorithm is now implemented on a field programmable gate array to provide fast lifetime estimates from a 32 × 32 low dark count 0.13 μm complementary metal-oxide-semiconductor single-photon avalanche diode (SPAD) plus time-to-digital conv...

Methods The intensification study with an entry inhibitor (maraviroc-MVC) included patients with stable antiretroviral therapy (ART) with three or more drugs, viral load (VL) of <50 copies/ml for two or more years, CD4 count of >350 cells/mm and CCR5-tropism. Latently infected resting CD4 cells were determined at baseline, week 12 and week 24, with further analyses planned at week 36 and week 4...