A reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed to analyze various fatty acids in recombinant mouse L cells. These fatty acids were the metabolites of oleic acid. A process was developed to extract fatty acids from the cell samples before RP-HPLC analysis. The samples were first saponified w~th 0.5 M NaOH in 96% ethanol then extracted with acidified ethyl ...

BACKGROUND Xuesaitong (XST) injection, consisting of total saponins from Panax notoginseng, was widely used for the treatment of cardio- and cerebro-vascular diseases in China. This study develops a simple and global quality evaluation method for the quality control of XST. METHODS High performance liquid chromatography-ultraviolet detection (HPLC-UV) was used to identify and quantify the chr...

objective(s) developing and validating a simple, efficient, reproducible and economic reversed phase high performance liquid chromatographic (rp-hplc) method for the quantitative determination of pefloxacin in bulk material, tablets and in human plasma. materials and methods a shim-pack clc-ods column and a mobile phase constituting acetonitrile: 0.025 m phosphoric acid solution (13:87 v/v, ph ...

Liquid-liquid extraction and solid phase followed by high-performance liquid chromatography (HPLC) connected with ultraviolet (UV) detection were used for the determination of phytohormones. The parameters influencing performance HPLC-UV method, including composition mobile gradient elution, column temperature, flow rate, wavelength, optimized. This method can simultaneously determine 11 phytoh...

Recently, a new universal HPLC detection technology has been developed based on charged aerosol detection that has led to the development of a universal HPLC detector, the Corona CAD. To further understand the capabilities of charged aerosol detection and the use of the technology in an HPLC detector, the Corona CAD was tested for its sensitivity, dynamic range, response from compounds of diffe...

A multicomponent pharmaceutical that contains loratadine, paracetamol, and pseudoephedrine was quantified using HPLC-PDA. The three analytes were well-separated in the dosage form on a C-18 column gradient mobile phase. quality by design strategy followed to achieve challenging separation. Screening optimization steps carried out investigate effect of many factors studied responses with minimum...

introduction: the mechanism of action and metabolism of clozapine, a complex drug used in psychopharmacology, is not completely understood. the effects of this compound in human body are related to some interactions with serotonin and dopamine neurotransmitter receptors. to further elucidate the mechanism of action and to support ongoing drug metabolism studies, [14c] clozapine with suitable ra...

Quantification of substances such as drug impurities or library compounds when pure standards are unavailable is difficult yet often necessary. This is often accomplished by HPLC based on relative response using low wavelength UV detection. The dependence of response on the optical properties of each component can lead to large errors in estimated quantity. Charged aerosol detection is a mass s...

Sensitive and rapid HPLC procedure was developed for determination of Indacaterol Maleate (IND) in bulk powder and capsules using Ultraviolet and fluorescence detection. The chromatographic separation was performed on C18 column as a stationary phase, and the mobile phase consisted of acetonitrile: 5mM acid hydrogen orthophosphate containing 0.3 % triethylamine (TEA) in ratio of (40: 60% v/v) a...