Novel, highly positively charged tripodal polyamines with appended heterocyclic moieties revealed an intriguing panel of protonation species within the biologically relevant range. Studied compounds bind nucleotide monophosphates by mostly electrostatic interactions but only the imidazole analogue showed selectivity toward UMP in respect to other nucleotides. Strong binding of all the studied c...

1Center for Orphan Drug Research, University of Minnesota, Minneapolis, MN, USA, 2 Department of Experimental and Clinical Pharmacology, College of Pharmacy, University of Minnesota, Minneapolis, MN, USA, 3 College of Science and Engineering, University of Minnesota, Minneapolis, MN, USA, 4 UMP MINCEP Epilepsy Care, Minneapolis, MN, USA, 5 Mayo Clinic, Rochester, MN, USA, 6 College of Veterinar...

A series of novel 5-substituted UDP-glucose derivatives with interesting fluorescent properties and potential applications as sensors for carbohydrate-active enzymes is reported. An efficient synthesis of the target molecules was developed, centred around the Suzuki-Miyaura reaction of (hetero)arylboronic acids with 5-iodo UDP-glucose. Interestingly, the optimised cross-coupling conditions coul...

We have considered in this paper the conditions for the equivalence of the counting rule and the likelihood ratio test when implementing the fusion rule of decisions corresponding to identically operated correlated sensors. A simple model of correlation, defined by two correlation indices, one for every hypothesis, has been considered. A main conclusion is that for properly operating sensors, t...

Formation of 5'-AMP, 5'-GMP, 5'-CMP and 5'UMP was confirmed in isolated rat liver mitochondria incubated with alpha-ketoglutarate, inorganic phosphate, purine nucleoside and pyrimidine nucleoside. Increased incorporation of 32Pi into ATP, GTP and UTP was observed by adding purine- and pyrimidine nucleosides. The phosphorylation of nucleosides was inhibited severely by arsenite and affected slig...

Erythrocytes from a patient with classical pyrimidine nucleotidase (PyN) deficiency had less than 10% residual PyN activity with uridine 5'-monophosphate (UMP) or cytidine 5'-monophosphate (CMP) as substrate, but exhibited brisk nucleotidase activity with thymidine 5'-monophosphate (dTMP). This strongly suggests the existence of separate enzymes or isozymes of PyN in normal human erythrocytes--...

We consider the problem of detecting visual evoked potentials (VEP's). A matched subspace lter is applied to the detection of the VEP and is demonstrated to perform better than a number of other evoked potential (EP) detectors. Unlike single-harmonic detectors, the MSF detector is suitable for detecting multi-harmonic VEP's. Moreover, the MSF is optimal in the uniformly most powerful (UMP) sens...