Members of the genera Desulfuromonas and Dehalococcoides reductively dechlorinate tetrachloroethene (PCE) and trichloroethene. Two primer pairs specific to hypervariable regions of the 16S rRNA genes of the Dehalococcoides group (comprising Dehalococcoides ethenogenes and Dehalococcoides sp. strain FL2) and the acetate-oxidizing, PCE-dechlorinating Desulfuromonas group (comprising Desulfuromona...

The next generation sequencing revolution has enabled rapid discovery of genetic markers, however, development of fully functioning new markers still requires a long and costly process of marker validation. This study reports a rapid and economical approach for the validation and deployment of polymorphic microsatellite markers obtained from a 454 pyrosequencing library of Atlantic cod, Gadus m...

Polychlorinated biphenyls (PCBs) accumulate and persist in sediments posing a risk to human health and the environment. Highly chlorinated PCBs are reductively dechlorinated in anaerobic sediments and two bacteria, designated o-17 and DF-1, from a novel phylogenetic group that reductively dechlorinate PCBs have recently been identified. However, there is a paucity of knowledge about the distrib...

INTRODUCTION The aim of the study is to evaluate interfacial bond strength between silicone prosthetic elastomers and polymethylmethacrylate (PMMA). MATERIALS AND METHODS Silicone elastomers were attached to PMMA and a total 120 specimens were fabricated which were then subdivided into 12 sub-groups. Each sample was then subjected to laboratory test to determine the bond strength. The specime...

Ten universal primer pairs of the plant chloroplast genome were used to amplify the chloroplast DNA (cpDNA) non-coding regions in eight mulberry (Morus spp.) genotypes, including M. mongolica, M. bombycis, M. alba, M. atropurpurea and M. multicaulis. Subsequently, the polymerase chain reaction (PCR) products were digested by seven restriction enzymes and the trnD-trnT fragment for sequence alig...

Arcidae species are commercially important bivalves in Japan and are commonly referred to as bloody ark due to their red blood. They have thick shells with distinct radiating ribs, and the numbers of these ribs are important morphological features for species discrimination. However, some Arcidae species are morphologically indistinguishable, with a similar number of the ribs in adults and defi...

background: echinocuccus granulosus, the causative agent of cystic echinococcosis has long been recognized as having a high degree of genetic divergence. the strains characterization seems to be essential for the establishment of a preventive and control strategy in every endemic area. using dna based methods for strain /genotype characterizations of e. granulosus have some difficulties, especi...

Amaç: Bu çalışmanın amacı, farklı plazma tipleri (argon ve oksijen), primer kumlama uygulamalarının monolitik zirkonya ile self-adeziv rezin siman arasındaki makaslama bağlanma dayanımına etkisinin incelenmesidir. Gereç Yöntemler: Elli adet disk hazırlandı sinterlendi. Zirkonya diskler uygulanacak yüzey işlemine göre rastgele beş gruba ayrıldı: Kontrol (herhangi bir işlemi uygulanmadı), Kumlama...

Akihiro Yoshida et al. (4). recently published an interesting paper describing the development of a 5 fluorogenic nucleasebased real-time PCR assay for quantitative detection of the oral pathogens Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. The authors provide the description of a set of primers and probe for amplification and detection of a broad range of bacteria based ...

Akihiro Yoshida et al. (4). recently published an interesting paper describing the development of a 5 fluorogenic nucleasebased real-time PCR assay for quantitative detection of the oral pathogens Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. The authors provide the description of a set of primers and probe for amplification and detection of a broad range of bacteria based ...