The mediated transport of citrate in Aerobacter aerogenes was studied. According to data obtained by examining the distribution of radioactive citrate at room temperature and at 0 C, a carrier system appears to be located on the membrane. The carrier system is inducible and very specific, not acting on the related compounds isocitrate and cis-aconitate. Induction required synthesis of both ribo...

The nucleoids of Escherichia coli, independently of the physiological state of the bacteria, are shown to be preserved as a fine-stranded fibrillar nucleoplasm by an OsO(4) fixation under defined conditions: acetate-veronal buffer pH 6, presence of Ca(++) and amino acids, stabilization with uranyl-acetate before dehydration. The same fixation procedure applied to the DNA of vegetative phage rev...

Employing a newly developed uranyl photofootprinting technique (Nielsen et al. (1988) FEBS Lett. 235, 122), we have analyzed the structure of the E. coli RNA polymerase deoP1 promoter open complex. The results show strong polymerase DNA backbone contacts in the -40, -10, and most notably in the +10 region. These results suggest that unwinding of the -12 to +3 region of the promoter in the open ...

We explored mechanisms for increasing U(VI) sorption by pretreating alumina surfaces with arsenate, which has a high affinity for binding with uranyl and is an analog for phosphate. Batch experiments were conducted at pH approximately 4 by pretreating a gamma-alumina surface with arsenate, followed by the addition of uranyl. Parallel experiments were conducted with different alumina loadings as...

The use of micelles to transpose lipophilic receptors, such as uranyl-salophen complexes, into an aqueous environment is a valuable and versatile tool. Receptor 1 incorporated into CTABr micelles forms a supramolecular system that exhibits excellent binding properties towards fluoride in water, despite the competition of the aqueous medium. To fully evaluate the potential of micellar nanodevice...

The chemistry of the actinide family is surprisingly rich. In order to better understand the affinity of specific chelates as in the case of ionic selective recognition or uptake by specific biomolecules, it is essential to better understand the intramolecular interactions. Although this has long been done for widely investigated transition metals, very few studies have been devoted to complexa...

The distribution of uranium in mice following intravenous and intraperitoneal injections of uranyl nitrate and uranyl tricarbonate was studied by autoradiographic and fluorometric methods at different times after administration. The investigations revealed a higher retention of uranium in the spleen and bone than in other organs, including the kidneys. We have been able to show that even very s...

A route for systematically accessing the oxo atoms of linear uranyl (UO22+) cation via cation–cation and halogen bonding interactions is detailed, interaction strengths are probed structural, vibrational, computational means.

Two electron microscopic staining techniques, one using tannic acid-glutaraldehyde as a fixative, and the other using tannic acid-uranyl acetate solution as a stain on ultra-thin sections of glutaraldehyde fixed material, were directly compared for elastic fibre staining on several human and animal tissues. Various concentrations of tannic acid were compared using both techniques. The two techn...