Introduction Many molecular biology applications require the determination of ss-, dsDNA, or RNA concentration in various samples. Most commonly, UV spectroscopy or Fluorescence spectroscopy is the preferred method of choice. Most simple and most convenient is UV spectroscopy, because nucleic acids have a characteristic UV absorbance spectrum, which can be used or quantitation without further s...

UV-Resonance Raman (UV-RR) spectroscopy with laser beam excitation source of 325 nm was applied to a solid-state study of chromophores in Eucalyptus globulus kraft pulps. The amount of chromophore structures in pre-bleached/fully bleached pulps absorbing at 325 nm linearly correlated with signal at ca 1600 cm in UV-RR spectra. The characteristic vibrations from particular pulp chromophore struc...

A compact UV pulsed laser pumped by high-power two-dimensional arrays of vertical-cavity surface-emitting lasers (VCSELs) was presented. A passively Q-switched 1064-nm Nd:YAG laser was side-pumped by kW-class 808-nm VCSEL pump modules and the output pulses were frequency quadrupled to 266-nm. 10-ns, 0.68-mJ UV pulsed output was obtained at 1.33k-Hz repetition rate. The average UV power is > 0.9...

In this study, novel carbohydrate-based nanoconjugates combining chemically modified chitosan with semiconductor quantum dots (QDs) were designed and synthesised via single-step aqueous route at room temperature. Glycol chitosan (G-CHI) was used as the capping ligand aiming to improve the water solubility of the nanoconjugates to produce stable and biocompatible colloidal systems. UV-visible (U...

The report describes the synthesis, self-association and DNA binding studies of an aromatic tripeptide H-Phe-Phe-Phe-OH (FFF). The peptide backbone adopts β-sheet conformation both in solid and solution. In aqueous solution, FFF self-assembles to form nanostructured aggregates. Interactions of this peptide with calf-thymus DNA (ct-DNA) have been studied using various biophysical techniques incl...

Extracellular UV-screening pigments gloeocapsin and scytonemin present in the exopolysaccharide (EPS) envelopes of extremophilic cyanobacteria of freshwater and marine environments were studied by Raman spectroscopy and compared to their intracellular photosynthetic pigments. This Raman spectral analysis of the extracellular pigment gloeocapsin showed that it shared Raman spectral signatures wi...

We describe the design and realization of a scheme for uv laser spectroscopy of singly-ionized iron (Fe II) with very high resolution. A buffer-gas cooled laser ablation source is used to provide a plasma close to room temperature with a high density of Fe II. We combine this with a scheme for pulsed-laser saturation spectroscopy to yield sub-Doppler resolution. In a demonstration experiment, w...

cu2o nanoparticles were synthesized by thermal treatment in liquid paraffin without any inert gas protection using nano structures of schiff base copper (ii) complex (1) as precursor. liquid paraffin was used as solvent and reductant. span 80 was applied to control the morphology of cuprous oxide nanoparticles. the nano structure of the complex was characterized by x-ray diffraction measurement...

Crystallinities of high-density polyethylene (HDPE) films containing various amounts of an oxo-biodegradable additive (HES-W) were investigated immediately after preparation and 6 weeks after ultraviolet (UV) irradiation (λ=254 nm). HDPE granules were mixed with oxo-biodegradable masterbatch in a twin-screw extruder and the extrudates were converted into films with thicknesses of 35±5 micromete...