volvement of the respiratory tract in a Dutch child. Clin Exp lmmunol 1971;9: 625-32. 5. Faux JA, Cram JD, Rosen FS, Merler E. An alpha heavy chain abnormality in a child with hypogammaglobulinemia. Cliii Immunol Immunopathol 1973;l:282-6. 6. Keren DF. High resolution electrophoresis and iminunoflxation. Techniques and interpretation. Boston, MA: Butterworth Publishers, 1987. 7. Solomon A, Kunk...

The purpose of this work was to study the isolation and a light microscopy technique for cultured lymphocytes. Blood samples were obtained by venipuncture with an anticoagulant added and centrifuged in a Percoll density gradient to separate the leukocytes. Lymphocytes were placed in 25 cm tissue culture flasks at 37°C. After culturing, they were fixed and stained with the methods used for blood...

BACKGROUND Although phlebotomy is a common procedure, there is limited information concerning to documented complications of venipuncture. CASE PRESENTATION A 45 year old left- handed woman was refered for elecrodiagnostic study with dysesthesia and pain in left medial forearm. She noted these symptoms three weeks after phelebotomy. Electrodiagnostic study showed severe involvement of left si...

It is human nature, perhaps more so for doctors and nurses, to comfort children in distress. Unfortunately, however, necessary medical procedures carried out by healthcare providers (eg, venipuncture) can often result in pain and discomfort, which may in turn cause the provider a degree of guilt and distress. Is it ever acceptable to comfort a paediatric patient with a kiss? This essay examines...

Of 45 individuals seropositive for human T cell lymphotropic virus type III/lymphadenopathy-associated virus, 45 were found to have detectable salivary antibodies to viral antigens by a radioimmunoprecipitation assay. The results also showed that a Western blot assay for salivary antibodies may be possible. The feasibility of a diagnostic test for human T cell lymphotropic virus type III/lympha...

In November 2002, the U.S. Food and Drug Administration approved the OraQuick Rapid HIV-1 Antibody Test (OraSure Technologies, Inc., Bethlehem, PA), a reliable, rapid test that detects HIV-1 antibodies. In contrast to other HIV-testing techniques, the OraQuick test uses blood obtained by finger stick instead of venipuncture and provides results in 20 minutes. Implications for practice, as well ...

Eosinophils are granulocytes integral to allergic inflammation and parasitic responses and comprise 1-4 % of the circulating leukocytes in human beings under normal conditions. Isolation of human eosinophils allows for ex vivo and in vitro experimentation, providing a valuable tool for the study of allergic mechanisms. Here, we describe a technique for the isolation of human eosinophils by nega...

A complete knot occurred in a 7F flow-directed balloon catheter inserted through the right internal jugular vein. The patient was intubated and placed under positive pressure ventilation. After gaining surgical exposure of the venipuncture site, a purse-string of Prolene 4--0 suture was placed around it. The patient was placed in the Trendelenburg position and the knotted catheter withdrawn, wh...

Introduction Blood collection in a pediatric population is time-consuming activity and an unpleasant experience. Moreover, many laboratory tests require only small amounts of blood while larger quantity usually drawn, generating excess waste that must be properly disposed of. To solve patient concerns workflow inefficiencies biomedical companies developed Pain-Free Draw (PFBD) devices. The aim ...