Mitotic root micronucleus (MCN) frequency in Vicia faba as a bioassay, is primarily based on the extent of the sentinel Vicia response in terms of cytogenetic damage quantitatively or qualitatively to indicate the presence of mutagenic contaminants. This paper describes an investigation designed to assess changes in MCN frequencies of Vicia faba from three generation plants obtained, respective...

The present study investigate the effect of Kochia indica extract using Vicia faba L assay. All parameters investigated were being affected. Four concentrations of 10, 20, 30 and 40% were applied for three time duration (6, 12, 24 h). The percentage of germination and plant height are dose and time dependent, probably due to alterations in cell cycle. Treated roots were highly affected compared...

Intracellular sucrose compartmentation in the palisade parenchyma of Vicia faba L. leaflets was investigated by comparing the specific radioactivity of photosynthetically labeled [(14)C]sucrose in samples enriched in vacuole to that in samples enriched in cytoplasm. Brief centrifugation of leaflet punches was used to sediment most of the palisade parenchyma cytoplasm in the adaxial ends of the ...

Metabolism of abscisic acid (ABA) was investigated in isolated guard cells and in mesophyll tissue of Vicia faba L. and Commelina communis L. After incubation in buffer containing [G-(3)H]+/-ABA, the tissue was extracted by grinding and the metabolites separated by thin layer chromatography. Guard cells of Commelina metabolized ABA to phaseic acid (PA), dihydrophaseic acid (DPA), and alkali lab...

Experiments were conducted over 2 years to quantify the response of faba bean (Vicia faba L.) to heat stress. Potted winter faba bean plants (cv. Wizard) were exposed to temperature treatments (18/10; 22/14; 26/18; 30/22; 34/26 °C day/night) for 5 days during floral development and anthesis. Developmental stages of all flowers were scored prior to stress, plants were grown in exclusion from ins...

Copper (Cu) toxicity on plants has become a major problem with increasing agricultural and environmental pollution. The effect of Cu on NADPH-diaphorase (NADPH-d) activity, commonly employed as a marker for nitric oxide synthase (NOS) activity and nitric oxide (NO) production, was investigated in root tips of Vicia faba L. We found that Cu stress stunted root growth and development of root hair...