Previous studies have shown that WRKY transcription factors play important roles in abiotic stress responses. Thus, virus-induced gene silencing (VIGS) was used to identify the function of SlWRKY79 salt tolerance tomato plants by downregulating expression gene. Under same treatment conditions, SlWRKY79-silenced showed faster stem wilting and more severe leaf shrinkage than control plants, bendi...

Virus-induced gene silencing has been suggested as a powerful and rapid technique for analysis of gene function in plant growth and development (Baulcombe, 1999). Infecting Nicotiana benthamiana with Tobacco Rattle Virus (TRV) containing a fragment of the gene encoding phytoene desaturase, an essential enzyme in carotene synthesis, results in a photo-bleached phenotype in photosynthetic tissues...

Gene silencing is an important tool in the study of gene function. Virus-induced gene silencing (VIGS) and hairpin RNA interference (hpRNAi), both of which rely on small interfering RNAs, together with artificial microRNAs (amiRNA), are amongst the most popular methods for reduction of gene activity in plants. However, all three approaches have limitations. Here, we introduce miRNA-induced gene...

The transition from vegetative growth to floral meristems in higher plants is regulated through the integration of internal cues and environmental signals. We were interested to examine the molecular mechanism of flowering in the day-neutral plant tomato (Solanum lycopersicum L.) and the effect of environmental conditions on tomato flowering. Analysis of the tomato genome uncovered 13 PEBP (pho...

Ethylene-responsive factors (ERF) are usually considered to play diverse roles in plant response to biotic and abiotic stresses. In this study, an ERF gene CaPTI1 was isolated from pepper transcriptome database. CaPTI1 contains an open reading frame (ORF) of 543 bp, which encodes a putative polypeptide of 180 amino acids with a theoretical molecular weight of 20.30 kDa. Results of expression pr...