Recently, there has been increased interest in equine viral arteritis (EVA) among veterinarians and horse owners. Outbreaks of the disease were identified initially in New Mexico, USA in 2006, and in the Normandy region of France in the summer of 2007. Both occurrences were associated with AI of cool-shipped semen. Each was linked to respiratory illness, neonatal death, abortion, development of...

Novel aerosol therapeutic modalities have been investigated for lung cancer. Inhaled gene therapy has presented safety and effectiveness previously in cystic fibrosis. However, safety concerns have been raised regarding the safety of non-viral vectors for inhaled gene therapy in lung cancer, and therefore small steps have been made towards this multifunctional treatment modality. During the las...

Targeted gene delivery systems have attracted great attention due to their potential in directing the therapeutic genes to the target cells. However, due to their low efficiency, most of the successful applications of polymeric vectors have been focused on genes which can achieve robust expression. Minicircle DNA (mcDNA) is a powerful candidate in terms of improving gene expression and prolongi...

Targeted gene delivery systems have attracted great attention due to their potential in directing the therapeutic genes to the target cells. However, due to their low efficiency, most of the successful applications of polymeric vectors have been focused on genes which can achieve robust expression. Minicircle DNA (mcDNA) is a powerful candidate in terms of improving gene expression and prolongi...

reye's syndrome is defined as fatty liver degeneration and acute encephalopathy following diffused abnormal mitochondrial activity. we reported a two year old girl admitted with lethargy and meningismus despite no evidence of blood sugar decrease, history of previous viral disease or drug use, complete tests showed reye syndrome as the final diagnosis. conservative protocols and treatment inclu...

elevated serum level of bilirubin is a common manifestation which is occurred in several diseases. hyperbilirubinemia can manifest either conjugated or unconjugated. conjugated or direct hyperbilirubinemia usually are caused by hepatocellular diseases or cholestatic liver diseases. merely conjugated hyperbilirubinemia is the main manifestation of two congenital syndromes, including dubin-johnso...

background: hepatitis activity index (hai) and fibrosing stage are two important findings during the evaluation of liver samples in patients with chronic hepatitis b. the aim of this study was to determine the hai and fibrosing stage in patients with anti-hbe positive chronic hepatitis b. methods: liver biopsy slides of 72 patients were evaluated at the department of pathology in two teaching h...

conclusions it is concluded that most hbeag negative hepatitis b patients with serum hbv dna level of 2000-20000 iu/ml, persistent normal alt concentration, and no or mild liver damage on biopsy can be clinically managed as hbv inactive carriers. results two hundred forty-three hbeag negative hepatitis b patients were stratified into three groups based on to their hbv dna level including group ...

a rapid and sensitive reverse transcription polymerase chain reaction (rt-pcr) and nested-pcr were used to detect bovine viral diarrhea virus 1 (bvdv-1) in bull semen. selected primers could amplify a part of the 5´utr of the bvdv genome. a 294 bp dna fragment was amplified and specificity of the results was confirmed by direct sequencing of the pcr product. prior to rna extraction, the seminal...

objective: dna vaccines have been widely used to develop immunity against various pathogens including parasites and viruses. the potential of dna vaccine to induce an effective immune response is related to the expression levels of the encoded protein in eukaryotic cells. therefore, optimization of plasmid dna delivery system is a major concern in protein expression in order to make an efficien...