Triacylglycerol (TAG) composition in vegetable oils was collaboratively analyzed in 9 laboratories using high-performance liquid chromatography (HPLC) equipped with a refractive index detector. Dococyl (C22) and triacontyl (C30) silica columns were used for analysis. The TAG molecular species in soybean, rapeseed, and palm oils were individually separated on the chromatogram. The collaborative ...

A simple reversed-phase chiral HPLC method has been developed and validated for direct separation of the enantiomers of zaltoprofen. Separation of the enantiomers was tested on numerous commercial chiral HPLC columns. Separation was best (resolution, RS = 3) on a Chiralcel OJ-RH stationary phase. Typical retention times of the S and R enantiomers were approximately 14 and 16 min, respectively. ...

Ergönül P.G., Nergiz C. (2010): Determination of organic acids in olive fruit by HPLC. Czech J. Food Sci., 28: 202–205. Organic acids (oxalic, citric, malic, and succinic) contents of Domat, Memecik and Uslu varieties of olives grown in Turkey were investigated using HPLC method. Organic acids were extracted from olives with water-methanol mixture solution 75:25 (v/v) and were analysed through ...

In a previous communication [l] we reported a procedure for the serum determination of midazolam using gas chromatography (GC) with a nitrogenselective detector. GC with nitrogen-selective detection resulted in a selective procedure for the direct determination of midaxolam with a sensitivity of 50 ng/ml. We present, in this paper, a procedure for the direct determination of midazolam and its m...

Solubility measurements using chemiluminescent nitrogen detection (CLND) has advantages of reduced compound requirement and increased throughput compared to UV-spectrophotometric and HPLC-based measurements. CLND with direct flow injection was evaluated for the measurement of thermodynamic solubility to support drug discovery. The limit of quantitation (LOQ), accuracy, and day-to-day reproducib...

Forced degradation studies are used to validate analytical procedures for the drug substance and products. of Pimecrolimus its cream were performed under stressed alkaline, acidic, oxidative thermal conditions according ICH guidelines Q1A(R2). A sensitive stability indicating RP-HPLC method was purposed validated separation Desmethyl Pimecrolimus. The chromatographic achieved with Phenomenax Lu...

Competitive reverse-transcription PCR (RT-PCR) techniques for quantification of gene expression employ titrations in which the products of multiple PCRs must be separated, analyzed and quantified to compute gene expression in a single sample. We have employed a novel, ion-pair reversed-phase HPLC (IP-RP-HPLC) system to analyze and quantify RT-PCRs performed with mutant RNA internal standards. P...

Curcumin is the main compound in turmeric and has problems its stability. The analytical method used to determine curcumin levels by using high performance liquid Chromatography (HPLC) Photodiode Array Detector (PDA). HPLC a separation capability good reproducibility with low detection limit. purpose of this research that isolation curcumine determined HPLC-PDA been validated can be applied as ...

BACKGROUND Sequential injection chromatography (SIC) is a new alternative separation technology. It has some advantages over high performance liquid chromatography (HPLC) regarding simplicity, inexpensiveness, portability, ease of use, maintenance requirement and operation time. In contrast, SIC has had suffered from some limitations. RESULTS The current work involves four achievements. (a) O...