Isolation and purification of major outer membrane proteins (OMP) from the cell wall envelope of Brucella abortus S-99 were achieved by sonication, solubilization and membrane fractionation in the presence of non-ionic detergent (Tx-100) and lysozyme treatments, followed by ultracentrifugation. The crude OMP was treated with trypsin to free the preparation from any other protein contaminan...

Abstract The behavior of box-shaped columns under heating is investigated. For this purpose, the various sections thin-wall were modeled and verified in different temperature ranges by ABAQUS software. results research showed that increasing thickness leads to increase buckling stability column change. Since will be better than thinner climate change because modulus elasticity. solid have hollo...

The Coanda effect (the tendency of a jet stream to adhere to a boundary wall), and the relevant anatomy, may explain the location of ductal jets within the main pulmonary artery. With the usual insertion of the duct close to the left pulmonary artery, during right ventricular ejection, the ductal jet adheres to the left wall of the main pulmonary artery. When right ventricular ejection is absen...

in the construction process of a plastic concrete cut-off wall, concreting is usually performed using the termie pipe method. in this method, concreting is performed from the lowest point in the excavated trench (containing the slurry) using a funnel and the pipe connected to it. the concrete poured in the funnel gradually settles in the panel through the termie pipe which is beneath the slurry...