A method for the separation and fractionation of the major whey proteins from a whey protein concentrate (WPC80) by anion-exchange chromatography coupled to a Fast Protein Liquid Chromatography (FPLC) system is proposed. The method is based on the use of an ionic column (Mono Q) and a salt gradient elution by increasing the ionic strength of the elution buffer (Tris–HCl 20 mM plus 0 to 1 M NaCl...

this study prepared new edible composite film by blending kefiran with whey protein. the film-forming solutions used different ratios of kefiran to whey protein (70/30, 50/50, 30/70, 10/90) and were cast at room temperature. the effects of the addition of whey protein on the physical, mechanical and water-vapor permeability (wvp) properties of the film were investigated. it was found that an in...

Whey is a highly polluting by-product of cheese processing. However, it has valuable nutritional properties since rich and balanced source proteins amino acids. Therefore, broad range functional that can be exploited for diverse applications. Research shown how the enzymatic hydrolysis whey releases bioactive peptides. In present study, protein concentrate (WCP) was performed using purified Spo...

The simultaneous separation of bovine whey proteins [alpha-lactalbumin and beta-lactoglobulin (A+B)] and soybean proteins was performed, for the first time, by capillary electrophoresis. Different experimental conditions were tested. The most suitable consisted of 0.050 M phosphate buffer (pH 8) with 1 M urea and 1.2 mg/ml methylhydroxyethylcellulose, UV detection at 280 nm, 15 kV applied volta...

Eighteen Holstein dairy cows averaging 141 days in milk were used in a complete randomized design to determine the effects of feeding liquid acid whey (LAW) on milk production and composition. Animals received a diet consisting of berseem clover (Trifolium alexandrinum., L), wheat straw and concentrate (Control). Treatments were control and control plus 40 liters/cow/day of LAW. Daily individua...

Milk protein concentrate (MPC) manufacture utilizes ultrafiltration to produce partially or completely delactosed high protein dairy ingredients. A variety of products are available in the liquid or dried form and range in protein content on a dry basis from 56 to 85%. The protein in MPC is a mixture of native casein and whey protein at a concentration of 80 and 20% of the total protein, respec...