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Protein-protein interactions play key roles in protein function and the structural organization of a cell. A thorough description of these interactions should facilitate elucidation of cellular activities, targeted-drug design, and whole cell engineering. A large-scale comprehensive pull-down assay was performed using a His-tagged Escherichia coli ORF clone library. Of 4339 bait proteins tested...
FhuA (Mr 78,992, 714 amino acids), siderophore receptor for ferrichrome-iron in the outer membrane of Escherichia coli, was affinity tagged, rapidly purified, and crystallized. To obtain FhuA in quantities sufficient for crystallization, a hexahistidine tag was genetically inserted into the fhuA gene after amino acid 405, which resides in a known surface-exposed loop. Recombinant FhuA405.H6 was...
Ku is an ubiquitous nuclear heterodimeric protein consisting of p70 and p86 subunits that binds double-stranded DNA termini and associates with chromosomes in vivo. It was originally described as an autoantigen in patients with certain autoimmune diseases. The individual subunits of Ku have been difficult to isolate from human cells without denaturation and attempts to produce functional recomb...
The alpha1- and beta1-subunits of human soluble guanylate cyclase (sGC) were coexpressed in the Sf9 cells/baculovirus system. In addition to the native enzyme, constructs with hexahistidine tag at the amino and carboxyl termini of each subunit were coexpressed. This permitted the rapid and efficient purification of active recombinant enzyme on a nickel-affinity column. The enzyme has one heme p...
This article is an attempt to have an analytical study of the book of Ahmad Ibn Ali Ibn Abbas Najashi, one of the Shiite experts in the science of the transmission of hadith. Considering certain proofs and evidence it shows that The List of Names of the Shiite Authors was presented to the world of knowledge at that time not at the time of the life of its author, but rather many years after his ...
testing plays a vital role in any language teaching program. it allows teachers and stakeholders, including program administrators, parents, admissions officers and prospective employers to be assured that the learners are progressing according to an accepted standard (douglas, 2010). the problems currently facing language testers have both practical and theoretical implications but the first i...
This article describes the homicide pattern in Penang Island, Malaysia over a three-year period (2007-2009). 65 homicide autopsies were performed at the Department of Forensic Medicine, Penang Hospital over the study period. The homicide rates ranged from 0.01 to 0.09/1000 population, the highest being in the Indian ethnic group. The majority (37%) of victims were in the 20-39 years age group. ...
minoo ’s ahriman is a term for discovering ahriman in the world of minoo: a world where it’s creatures are invisible, undesirable, and without a body . in this article, minoo’s ahriman in the avesta and the pahlavi texts are surveyed. in gahan, ahriman exists as the evil nature as opposed to the good nature that leads to his creation in the haftha. in new avesta the minavi existence continues w...
Chemical Engineering, School of Engineering and Information Technology, Universiti Malaysia Sabah, UMS Road, 88400 Kota Kinabalu, Sabah, Malaysia *School of Chemical Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia **School of Dental Sciences, Health Campus, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia ***Malaysian Institute Pharmaceutical & Nutraceutical, MOSTI...
this article examines the goals of george w. bush’s middle east policy and its legacy in the region. it further gives a detailed analysis of three major dilemmas in us policy - iraq, iran, and lebanon - towards the region at the present time. it then examines barak obama’s declared positions on these issues during his election campaign and his possible course of action in the region as presiden...
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