Wound healing is a highly dynamic process over spatial and temporal scales. Key to wound outcomes are fibroblasts, whose precise functions in tissue repair only partially understood. We constructed an atlas of mouse skin using several published single-cell RNA-seq (scRNA-seq) datasets. Using scRNA-seq data integration across time, we identified key cell types states that both shared as well uni...

We have examined the role of protein kinase D1 (PKD1) signaling in intestinal epithelial cell migration. Wounding monolayer cultures of intestinal epithelial cell line IEC-18 or IEC-6 induced rapid PKD1 activation in the cells immediately adjacent to the wound edge, as judged by immunofluorescence microscopy with an antibody that detects the phosphorylated state of PKD1 at Ser(916), an autophos...

Rao JN, Rathor N, Zou T, Liu L, Xiao L, Yu T, Cui Y, Wang JY. STIM1 translocation to the plasma membrane enhances intestinal epithelial restitution by inducing TRPC1-mediated Ca signaling after wounding. Am J Physiol Cell Physiol 299: C579–C588, 2010. First published July 14, 2010; doi:10.1152/ajpcell.00066.2010.—Early epithelial restitution is an important repair modality in the gut mucosa and...

This paper is an attempt to develop a notion of violence based on the ability wound slowly. It starts by exploring everyday events in strangulated West Bank site struggling with Israel's colonisation and settlement policies, thus painting nuanced picture how slowness slow proceedings through unspectacular mundane spatialisations. The spatially manifold “slow wounding” offered, one that not only...

Rathor N, Zhuang R, Wang JY, Donahue JM, Turner DJ, Rao JN. Src-mediated caveolin-1 phosphorylation regulates intestinal epithelial restitution by altering Ca influx after wounding. Am J Physiol Gastrointest Liver Physiol 306: G650–G658, 2014. First published February 20, 2014; doi:10.1152/ajpgi.00003.2014.—Early mucosal restitution occurs as a consequence of intestinal epithelial cell (IEC) mi...

The purpose of this study was to elucidate the role of alpha-tocopherol succinate (TS)- and AMD3100-mobilized progenitors in mitigating combined injury associated with acute radiation exposure in combination with secondary physical wounding. CD2F1 mice were exposed to high doses of cobalt-60 gamma-radiation and then transfused intravenously with 5 million peripheral blood mononuclear cells (PBM...

PURPOSE To determine whether production and localization of beta 4 integrin is altered during in vivo corneal epithelial cell migration in response to debridement wounding. METHODS Rat corneas were wounded and animals were killed at times ranging from 3 hours to 14 days. At various time points, corneal epithelial integrins were quantitated by gel electrophoresis and immunoblotting of epitheli...

Wounding is one of many abiotic stresses that produce signals that propagate from injured into adjacent non-injured tissues and induce the de novo synthesis of specific wound-induced proteins. Some of these induced proteins are enzymes of phenolic metabolism, such as phenylalanine ammonia-lyase (PAL), whose increased activity leads to the accumulation of phenolic compounds (e.g., chlorogenic ac...

The mitogen-activated protein kinase (MAPK) phosphatases (MKPs) are negative regulators of MAPKs. In dicotyledons such as Arabidopsis and tobacco, MKPs have been shown to play pivotal roles in abiotic stress responses, hormone responses and microtubule organization. However, little is known about the role of MKPs in monocotyledons such as rice. Database searches identified five putative MKPs in...