This thesis focuses on the evaluation of growth factors, cytokines and chemokines present in the wound environment of burn, chronic and surgical wounds. The first chapters describe a differential expression of these mediators in wound exudates isolated from the 3 different wound types. Our results indicate that development of a universal therapy for these wounds is not an option. Since surgical...

This study was designed to assess the efficacy of vacuum sealing drainage (VSD) on skull exposure wounds in rabbits and to investigate the underlying mechanism of the process. Full-thickness excisional circular wounds 2×2 cm with or without periosteum involvement were created in 88 New Zealand white rabbits (mean body weight: 3.0±0.65 kg). Animals were randomly divided into 4 groups: periosteum...

Wound healing in horses is complicated, particularly when wounds are on the limb. The objectives of this study were to clone equine thrombospondin II (THBS2) and secreted protein acidic and cysteine-rich (SPARC) cDNAs and to compare the spatiotemporal expression of mRNAs and proteins during repair of body and limb wounds. These molecules were targeted in view of their potential biological contr...

INTRODUCTION This case series aims to study the effectiveness of Renasys-GO™ negative pressure wound therapy system in the healing of diabetic lower limb ulcers. MATERIALS AND METHODS An electronic vacuum pump (Renasys-GO™, Smith & Nephew GmbH) was used to apply negative pressure wound therapy on wounds, with pressure settings determined according to clinical indication. Changes in wound dime...

Brown recluse spider (Loxosceles) bites cause lesions ranging from chronic necrotic ulcers to acute life-threatening sepsis. Based on our experience in treating acute and chronic wounds with negative pressure, we postulated that vacuum-assisted closure (VAC) would be valuable in this application. Chester pigs were procured and injected with purified brown recluse spider venom, 1 µl of venom in ...

Chronic wounds in skin persist because the normal process of wound healing is obstructed. Failures occur in the inflammation and proliferation phases of wound healing that reduce formation of granulation tissue and prevent epithelial migration to close the wound, resulting mostly from vascular insufficiencies of multiple etiologies. Microbial contamination of chronic wounds also contributes imp...

Background: To assess the effectiveness of vacuum assisted closure (VAC) therapy for healing of infected wounds. Methods: In this prospective case-control study, total of 25 patients with chronic non-healing wounds were recruited. Before the application of VAC, surgical debridement was performed to remove all the devitalized necrotic tissues as per requirement and the wound was packed with povo...

Sericin has good hydrophilic properties, compatibility, and biodegradation, it can be used as a wound-healing agent. We evaluated the effects of sericin on wound healing and wound size reduction using rats by generating two full-thickness skin wounds on the dorsum. Group 1 animals were treated with Betadine on left-side (control) wounds and, with 8% sericin cream on right-side (treated) wounds....