The development of strong and tunable promoter elements is necessary to enable metabolic and pathway engineering applications for any host organism. Here, we have expanded and generalized a hybrid promoter approach to produce libraries of high-expressing, tunable promoters in the nonconventional yeast Yarrowia lipolytica. These synthetic promoters are comprised of two modular components: the en...

Abstract Producing high value-added products from waste lipid feedstock by microbial cell factory has great advantages to minimize the pollution as well improve economic value of wasted oils and fats. Yarrowia lipolytica is a non-conventional oleaginous yeast can grow on variety hydrophobic substrates. In this study, we explored its ability synthesize α-farnesene, an important sesquiterpene, us...