OBJECTIVE The objective of this study was to develop a reproducible method of establishing the concentration of yeast cells per milliliter of solution. METHODS Three methods of determining the number of yeast cells in solution were compared: Neubauer's counting chamber, spectrophotometry, and nephelometry. RESULTS All three methods were comparable and reproducible. The following formulas we...

Human monocytes/macrophages (M psi) were infected with Histoplasma capsulatum yeast cells, and intracellular growth was quantified after 24 h of incubation in medium alone or in medium containing cytokines. Yeast cells multiplied within freshly isolated monocytes, cultured M psi, and alveolar M psi with intracellular generation times of 14.2 +/- 1.4, 18.5 +/- 2.1, and 19.9 +/- 1.9 h (mean +/- s...

This study reports the production of L-asparaginase (ASNase), an enzyme mainly used for treatment acute lymphoblastic leukemia, by Leucosporidiumscottii L115, a psychrotolerant yeast isolated from Antarctic ecosystem. Focus was given to select most appropriate medium components able maximize this yeast, as first step development new process produce ASNase. By combining knowledge in bioprocesses...

Microbial fuel cells convert the chemical energy conserved in organic matter wastewater directly to electrical through living microorganisms. These devices are environmentally friendly thanks their ability simultaneously produce and treatment. The output power of yeast microbial cell (YMFC) depends mainly on glucose concentration glucose/yeast ratio. Thus, paper aims boost YMFC by identifying b...

As the population grows, there is a need to address continuous depletion of non-renewable energy sources and their negative effects on environment. This led substantial assessment possible innovations raw materials increase volumetric productivity alternative fuels supply needed worldwide. In addition its environment-friendly properties, biofuel derived from plant-based also sustainable materia...

The enucleation of yeast cells is of interest for the production (by fusion) of special industrial yeast hybrids, possessing mixed cytoplasms (cybrids) but only one nucleus. Such cybrids could be designed to have a modified phenotype without change of ploidy. However, no methods for the enucleation of yeast cells have yet been reported. In animal cells, enucleation usually uses the cytochalasin...

The effect of thermally processed rice koji extracts on survival of yeast Saccharomyces cerevisiae was examined in comparison with non-heated koji extract. In chronological life span (CLS) tests on high-sugar fermentation, the survivals of the yeast cells grown with heated koji extracts were higher than non-heated koji extract. Heat treatment of the extracts by autoclaving led to a loss in most...

Actin branches out in yeast any of the same proteins control actin polymerization in budding yeast and mammalian cells. But budding yeast researchers—hampered by an emphasis on genetics and an inaccessible yeast cytoplasm dense with ribosomes and glycogen granules—have often lagged behind in providing structural descriptions based on electron microscopy (EM). This has fueled suspicions about wh...

Actin branches out in yeast any of the same proteins control actin polymerization in budding yeast and mammalian cells. But budding yeast researchers—hampered by an emphasis on genetics and an inaccessible yeast cytoplasm dense with ribosomes and glycogen granules—have often lagged behind in providing structural descriptions based on electron microscopy (EM). This has fueled suspicions about wh...

The present study was carried out to evaluate the effect of phagocytosis of killed yeast on the induction of apoptosis in two human solid tumors of the gastrointestinal (GI): the squamous cell carcinomas of the tongue (SCCA) are (SCC-4 and SCC-9) and the adenocarcinomas of the colon (ADENOCA) are (Caco-2 and DLD-1). Cancer cells were cultured with heat killed Saccharomyces cerevisiae, baker's a...