EULER & ADLER [1935] recently described the preparation from yeast of a cell-free enzyme, "heterophosphatese" which catalyses the transference of phosphate from (muscle) adenosinetriphosphate to glucose or fructose with the formation of a hexosephosphate. Later Meyerhof [1935] showed that preparations of hexokinase contain "heterophosphatese". The natural inference from these experiments is tha...

enzymes on oX-D-glucosyl phosphate also yields a branched polysaccharide, although Q-enzyme alone has no action on the glucoside. In the animal kingdom a related enzyme system may be partly responsible for glycogen synthesis. Muscle phosphorylase synthesizes amylose-type chains from OC-D-glucosyl phosphate and the concurrent action of this enzyme and an enzyme preparation (branching factor or s...

Eduard Buchner’s discovery of cell-free metabolism 100 years ago was a landmark in biochemistry; it can be said to be the start of detailed metabolic studies. The fact that a soluble fraction of yeast, which he called “zymase”, was able to produce ethanol from glucose was revolutionary, and finally put paid to the idea that living cells were essential for fermentation (Buchner, 1897). Later, th...

Yeast and vertebrate nuclear pores display significant morphological similarity by electron microscopy, but sequence similarity between the respective proteins has been more difficult to observe. Herein we have identified a vertebrate nucleoporin, Nup93, in both human and Xenopus that has proved to be an evolutionarily related homologue of the yeast nucleoporin Nic96p. Polyclonal antiserum to h...