Maize is subject to ear rot caused by toxigenic Aspergillus and Fusarium species, resulting in contamination with aflatoxins, fumonisins, trichothecenes, and zearalenone (ZEN). The trichothecene group and ZEN mycotoxins are produced by the cereal pathogen Fusarium graminearum. A transgenic detoxification system for the elimination of ZEN was previously developed using an egfp::zhd101 gene (gfzh...

Lon W. Whitlow Department of Animal Science North Carolina State University Raleigh, NC 27695 Phone: 919-515-7602 Fax: 919-515-2152 Email: [email protected] Summary Considerable research has been directed at finding methods to prevent toxicity of mycotoxins. Some of the approaches have included mycotoxin separation from contaminated feeds, detoxification and inactivation. Detoxification and ...

Project Aquafluosense is designed to develop prototypes for a fluorescence-based instrumentation setup in situ measurements of several characteristic parameters water quality. In the scope project an enzyme-linked fluorescent immunoassay (ELFIA) method has been developed detection environmental xenobiotics, including mycotoxin zearalenone (ZON). ZON, produced by plant pathogenic Fusarium specie...

Abstract Liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) was used for the rapid quantification of multiple mycotoxins, specifically aflatoxins B 1 , 2 G and (AFB AFB AFG ), ochratoxin A (OTA), deoxynivalenol (DON), zearalenone (ZEN), in walnuts, pistachios, peanuts, coffee beans, rice, chickpeas from various countries. Total counts fungi, Aspergillus flavus, parasiticus were also asse...

With an increased knowledge of the mechanism action Fusarium mycotoxins, concept that these substances are deleterious only for monogastric species is obsolete. Indeed, most mycotoxins can be converted into less toxic compounds by rumen microflora from healthy animals. However, mycotoxin absorption and its conversion to more metabolites, as well their impact on immune response subsequently anim...

This study developed and validated an analytical methodology for the determination of aflatoxins, enniatins, beauvericin, zearalenone, ochratoxin-A, alternariols, HT-2 T-2 toxin in soy, oat, rice almond beverages, based on solid phase extraction columns (SPE) analyzed by liquid chromatography coupled to mass spectrometry tandem. C18 SPE was successfully applied, obtaining recoveries that range ...

Crops can be contaminated by fungi which produce mycotoxins. Many fungal strains are responsible for producing varied The research carried out so far has described over 400 different They have chemical and physical properties that significantly differ, they produced several existing fungi. intake of mycotoxins through food achieved directly, feeding on food, or indirectly from foods animal orig...

The efficacy of cold atmospheric pressure plasma (CAPP) with ambient air as working gas for the degradation of selected mycotoxins was studied. Deoxynivalenol, zearalenone, enniatins, fumonisin B1, and T2 toxin produced by Fusarium spp., sterigmatocystin produced by Aspergillus spp. and AAL toxin produced by Alternaria alternata were used. The kinetics of the decay of mycotoxins exposed to plas...

The efficacy of cloning a recombinant mycotoxin antibody in plants was tested using Arabidopsis as a model. An antizearalenone single-chain Fv (scFv) DNA fragment was first cloned in the newly constructed phage display vector (pEY.5) and then recloned in the plant transformation vector pKYLX71::35S(2). After transformation, constructs of antizearalenone scFv were introduced into immature Arabid...