the effect of a neuropeptide, the tachykinin on α-amylase, protease and lipase activities in the midgut of american cockroach, periplaneta americana (linnaeus) is evaluated . immunohistochemical reactivities against tachykinin (tachykinin- ir) were detected in the midgut. tachykinin-ir cells in three regions (anterior, median and posterior) of the midgut were counted in normally fed, starved fo...

The present review assesses the potential of the Phaseolus vulgaris a-amylase inhibitor isoform 1 (a-AI1) starch blockers as a widely used remedy against obesity and diabetes. Consumption of the a-amylase inhibitor causes marginal intraluminal a-amylase activity facilitated by the inhibitor’s appropriate structural, physico-chemical and functional properties. As a result there is decreased post...

Staphylococcus aureus is one of the prominent Gram positive human pathogen secretes many surface and secretary proteins including various enzymes and pathogenic factors that favour the successful colonization and infection of host tissue. α-amylase is one of the enzymes secreted by S. aureus which catalyses the breakdown of complex sugars to monosaccharides, which are required for colonization ...

BACKGROUND Starch, a homopolysaccharide is an important and an abundant food reserve and energy source. Starches are processed to yield different products which find many industrial applications. Alpha-amylases hydrolyze starch by cleaving α-1,4-glucosidic bonds and have been used in food, textile and pharmaceutical industries [Sun et al. 2010]. Enzymatic conversion of starch with amylase prese...

In the present study, selection of an appropriate volume and temperature for α-amylase production by Saccharomyces cerevisiae GCB-20 has been reported. The fermentation medium at the level of 15-40 ml was investigated in 250 ml conical flasks for enzyme production. Maximum α-amylase production (210 IU/ml/min) by S. cerevisiae GCB-20 was obtained in 25 ml of fermentation medium at 35°C. The kine...

Materials and Methods: Antidiabetic potential of GW was evaluated by assay of α-amylase and α-glucosidase inhibitory activity; haemoglobin glycosylation and yeast glucose uptake. The antioxidant potential of GW was assessed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) assay, 2,2′azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation decolorization assay and superoxide assay. The inh...

α-Amylases are widely used enzymes in starch saccharification and baking industries, and in textile desizing. These are endoenzymes which cleave α-1,4-glycosidic linkages randomly in starch, thus, generate maltose and malto oligosaccharides. The commercially available α-amylases have certain limitations like a limited activity at low pH and Ca2+dependence, therefore, the search for novel acid s...

We coupled a kinetic procedure to a selective inhibiting method for determining amylase isoenzymes in biological samples, using 4-nitrophenylmaltopentaoside plus 4-nitrophenylmaltohexaoside as substrate and a wheat-germ selective inhibitor with the Gilford S-III spectrophotometer. On plotting remaining amylase activities/total amylase activities (R/T) vs pancreatic amylase activities/salivary a...

BACKGROUND Cereal seed germination involves mobilization of storage reserves in the starchy endosperm to support seedling growth. In response to gibberellin produced by the embryo the aleurone layer synthesizes hydrolases that are secreted to the endosperm for degradation of storage products. In this study analysis of intracellular protein accumulation and release from barley aleurone layers is...