The inhibition of enzymes involved in the breakdown of carbohydrates is considered a therapeutic approach to the management of type-2 diabetes. This study sought to investigate the effects of essential oil from clove bud on α-amylase and α-glucosidase activities. Essential oil from clove bud was extracted by hydrodistillation, dried with anhydrous Na2SO4 and characterized using gas chromatograp...

Running title: α-glucoside-processing enzymes in Pyrococcus furiosus 20 ABSTRACT Bioinformatics analysis and transcriptional response information for Pyroccoccus furiosus grown on α-glucans led to identification of a novel isomaltase (PF0132) representing a new GH family, a novel GH57 β-amylase (PF0870), and an extracellular starch-binding protein (1,141 amino 5 acids) (PF1109-1110), in additio...

For digestion of starch in humans, α-amylase first hydrolyzes starch molecules to produce α-limit dextrins, followed by complete hydrolysis to glucose by the mucosal α-glucosidases in the small intestine. It is known that α-1,6 linkages in starch are hydrolyzed at a lower rate than are α-1,4 linkages. Here, to create designed slowly digestible carbohydrates, the structure of waxy corn starch (W...

objective(s):one strategy for the treatment of diabetes is inhibition of pancreatic α- amylase. plants contains different chemical constituents with potential for inhibition of α-amylase and hence maybe used as therapeutic. materials and methods: urtica dioica and juglans regia linn were tested for α-amylase inhibition. different concentrations of leaf aqueous extracts were incubated with enzym...

BACKGROUND Medicinal plants are becoming more popular in the treatment of various diseases because of the adverse effects of the current therapy, especially antioxidant plant components such as phenols and flavonoids have a protective role against oxidative stress-induced degenerative diseases like diabetes. Thus, the purpose of this study was to investigate β-cell protection and antidiabetic a...

We describe a simple and rapid method for measuring pancreatic and salivary type amylases using the Hitachi 705 analyser. The determination is based on the inhibition of salivary amylase using an inhibitor isolated from wheat germ. The precision of the proposed method was very good: within-day precision varied from 0.4 to 2.5% (CV) and day-to-day precision from 2.2 to 3.7% (CV). The new applica...

background and objective: in the present study, bacillus polymyxa ncim no. 2539 was selected to utilize agro-industrial byproduct (orange peel) for amylase production under submerged fermentation conditions. material and methods: different agro-industrial byproducts like cane molasses, wheat bran, rice bran and orange peel were screened for maximum amylase production. amylase activitiy of bacil...

1. Rabbit antisera to purified rat parotid alpha-amylase were prepared. 2. The relationships between rat parotid-gland, pancreatic, serum and liver amylase were investigated by using the antisera in immunodiffusion, immunoelectrophoresis and immunoinhibition experiments. 3. Serum and liver amylase were identical, and very similar to parotid-gland amylase; pancreatic amylase was, however, quite ...