نتایج جستجو برای: آزمون rflp
تعداد نتایج: 130676 فیلتر نتایج به سال:
OBJECTIVE Bacteremia due to lactobacilli is uncommon, yet it is increasing in frequency, especially among immunosuppressed patients. In the clinical laboratory, lactobacilli must be subcultured from positive blood cultures before identification by traditional biochemical methods. Delays in diagnosis are significant because the organisms are inherently resistant to vancomycin, a drug frequently ...
Genomic variability among isolates of Campylobacter jejuni from broiler flocks in the Czech Republic
The bacterium Campylobacter jejuni naturally colonizes the gastrointestinal tract of many birds and animals, resulting in a harmless commensal relationship, but this microbe can also promote pathogenesis in humans, resulting in a productive gastroenteritis leading to a mild bloody diarrhoeal syndrome. Consumption or handling of poultry meats is prevalent source of. C. jejuni for infection in hu...
A twenty-four year old male Peruvian of Japanese origin, who came to Japan in September 1990 and had been working in a minor factory in a rural area, was admitted to a hospital in March '91 with severe cough. Smear examination of his sputum smear was positive for acid-fast bacilli and his chest X-ray showed multiple cavities (Index case). Subsequent contact examination identified further four p...
In most Japanese populations of dojo loach (Misgurnus anguillicaudatus), gonochoristic diploids genetically diversified groups (A and B, further subdivided into B1 B2) are present, whereas unisexual clonal lineages inhabit certain localities in the Hokkaido Ishikawa Prefectures Japan. Through a series genetic studies including DNA markers, loaches were deemed to originate from hybridization eve...
Persistent human papillomavirus (HPV) infection is an essential factor of cervical cancer. This study evaluated the analytical performance of restriction fragment length polymorphism polymerase chain reaction (PCR-RFLP) assay compared to PapilloCheck® microarray to identify human papilloma virus (HPV) in cervical cells. Three hundred and twenty-five women were analyzed. One sample was used for ...
A cross-sectional study was carried out in the Ghibe valley from August to October 2010. 411 head of cattle were sampled in eight villages for buffy coat examination (BCE) and blood spots were collected from each animal for trypanosomose diagnosis by 18S-PCR-RFLP and diminazene aceturate (DA) resistance by Ade2-PCR-RFLP. Three villages were selected in a zone where trypanosomosis control operat...
Ecological diversity indices are frequently applied to molecular profiling methods, such as terminal restriction fragment length polymorphism (T-RFLP), in order to compare diversity among microbial communities. We performed simulations to determine whether diversity indices calculated from T-RFLP profiles could reflect the true diversity of the underlying communities despite potential analytica...
Abstract Background Emergence of fluoroquinolone resistance in gut pathogens is a cause concern. Resistant to quinolone mainly due the point mutations at quinolone-resistance determining regions (QRDR). The aim study was develop polymerase chain reaction-restriction fragment length polymorphism assay (PCR-RFLP) detect QRDR gyrA and gyrB enteric pathogens. Methodology PCR-RFLP done for 83 region...
Ghost crabs (genus Ocypode spp.) found on sandy beaches are susceptible with the loss of an ecotone, making them useful as indicators anthropogenic alteration, such seawall construction. On Sendai Bay Pacific coast Tohoku region northern Japan, three species have been sympatrically observed in recent years: O. stimpsoni, ceratophthalmus, and sinensis. However, these only occur from late summer ...
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