After injecting the cell body of the giant serotonergic neuron (GCN) in the cerebral ganglion of Aplysia with horseradish peroxidase (HRP), we found that some glial cells were labeled along the cerebrobuccal connective and posterior lip nerve, the two nerves that contain the major axon branches of the injected neuron. Starting at a distance of about 3 mm from the site of the injection, only gli...

This system built from commercially available parts and microfluidics allows for efficient robust readout of HRP/TMB based immunoassays.

We report here on a facile enzymatic polymerization protocol to prepare enzyme-poly(thiophene-3-boronic acid) (PTBA) polymeric biocomposites (PBCs) for high-performance mono-/bi-enzyme amperometric biosensing. Horseradish peroxidase (HRP)-catalyzed polymerization of thiophene-3-boronic acid (TBA) monomer was conducted in aqueous solution containing HRP (or plus glucose oxidase (GOx)) by either ...

We studied with morphometric methods the endocytosis by pheochromocytoma cells of a conjugate of wheat germ agglutinin with ferritin (WGA-Ft) and of horseradish peroxidase (HRP). Quantitative studies indicated that WGA-Ft was cleared slowly from cell surfaces and that it was not recycled to the surface. Cells labeled with WGA-Ft for 15 min at room temperature were washed and incubated in medium...

Polyaniline (PANI), an attractive conductive polymer, has been successfully applied in fabricating various types of enzyme-based biosensors. In this study, we have employed mesoporous silica SBA-15 to stably entrap horseradish peroxidase (HRP), and then deposited the loaded SBA-15 on the PANI modified platinum electrode to construct a GA/SBA-15(HRP)/PANI/Pt biosensor. The mesoporous structures ...

Horseradish peroxidase (HRP), a highly investigated member of the peroxidase family, has been known − among many other biological activities − to catalyze the oxidation of flavonoids and phenolic substrates overall, quercetin among them. On the other hand, quercetin is very well known for its expressed antioxidant activities, which in the case of UV external radiation can be expressed partly in...

Horseradish peroxidase (HRP) mediates efficient conversion of many phenolic contaminants and thus has potential applications for pollution control. Such potentially important applications suffer however from the fact that the enzyme becomes quickly inactivated during phenol oxidation and polymerization. The work here provides the first experimental data of heme consumption and iron releases to ...

A new signal amplification strategy based on simultaneous application of gold nanoparticles (AuNPs) and horseradish peroxidase (HRP) was employed to improve the sensitivity of an electrochemical immunoassay for detection of human IgG (hIgG), as a model antigenic protein. This immunoassay system was fabricated on magnetic carboxyl-functionalized multi-walled carbon nanotubes (COOH-MWCNT/Fe3...